Mouse Prostasin ELISA Kit PicoKine® | Boster EK1273 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse Prostasin ELISA Kit PicoKine®

catalog :

EK1273

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1273

Product Name :

Mouse Prostasin ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Mouse Prostasin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Prss8 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

PRSS8

Uniprot ID :

Q9ESD1

Immunogen :

Expression system for standard: NS0; Immunogen sequence: A30-Q289

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

0.78 ng/ml - 50 ng/ml

Sensitivity :

<50 pg/ml

Sample Type :

cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Prostasin

Synonyms :

Prostasin;3.4.21.-;Channel-activating protease 1;CAP1;Serine protease 8;Prostasin light chain;Prostasin heavy chain;Prss8;Cap1;

Protein Name :

Prostasin

Molecular Weight :

36729 MW

Protein Function :

Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates (By similarity). Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G).

Subcellular Localization :

Cell membrane ; Single-pass membrane protein .

Sequence Similarities :

Belongs to the peptidase S1 family.

Background :

Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

Research Category :

Cell Biology, Proteolysis / Ubiquitin, Proteolytic Enzymes