Human Prostasin ELISA Kit PicoKine® | Boster EK1272 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Prostasin ELISA Kit PicoKine®

catalog :

EK1272

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 1

Reference
Mok S, Chao J, Skates S, Wong K, Yiu G, Muto M, et al. Prostasin, a potential serum marker for ovarian cancer: identification through microarray technology. J Natl Cancer Inst. 2001;93:1458-64 pubmed

image

image 1 :

Human Prostasin PicoKine ELISA Kit standard curve

product information

SKU :

EK1272

Product Name :

Human Prostasin ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

369 USD

Description :

Human Prostasin ELISA Kit PicoKine® (96 Tests). Quantitate Human PRSS8 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

PRSS8

Uniprot ID :

Q16651

Immunogen :

Expression system for standard: CHO; Immunogen sequence: A33-G319

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

1.56 ng/ml - 100 ng/ml

Sensitivity :

<50 pg/ml

Sample Type :

cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Capture/Detection Antibodies :

polyclonal antibody from sheep, polyclonal antibody from sheep

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Prostasin

Synonyms :

Prostasin;3.4.21.-;Channel-activating protease 1;CAP1;Serine protease 8;Prostasin light chain;Prostasin heavy chain;PRSS8;

Protein Name :

Prostasin

Molecular Weight :

36431 MW

Protein Function :

Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G).

Subcellular Localization :

Prostasin: Cell membrane; Single-pass membrane protein.

Tissue Specificity :

Found in prostate, liver, salivary gland, kidney, lung, pancreas, colon, bronchus and renal proximal tubular cells. In the prostate gland it may be synthesized in epithelial cells, secreted into the ducts, and excreted into the seminal fluid.

Sequence Similarities :

Belongs to the peptidase S1 family.

Background :

Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

Research Category :

Cell Biology, Proteolysis / Ubiquitin, Proteolytic Enzymes

more info or order :

Boster product webpage