Human Ficolin-3 ELISA Kit PicoKine® | Boster EK1259 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Ficolin-3 ELISA Kit PicoKine®

catalog :

EK1259

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 2

Reference
Fust G, Munthe Fog L, Illes Z, Széplaki G, Molnar T, Pusch G, et al. Low ficolin-3 levels in early follow-up serum samples are associated with the severity and unfavorable outcome of acute ischemic stroke. J Neuroinflammation. 2011;8:185 pubmed publisher
Andersen T, Munthe Fog L, Garred P, Jacobsen S. Serum levels of ficolin-3 (Hakata antigen) in patients with systemic lupus erythematosus. J Rheumatol. 2009;36:757-9 pubmed publisher

image

image 1 :

Human Ficolin-3 PicoKine ELISA Kit standard curve

product information

SKU :

EK1259

Product Name :

Human Ficolin-3 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

369 USD

Description :

Human Ficolin-3 ELISA Kit PicoKine® (96 Tests). Quantitate Human FCN3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

FCN3

Uniprot ID :

O75636

Immunogen :

Expression system for standard: NS0; Immunogen sequence: Q24-R299

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

0.78 ng/ml - 50 ng/ml

Sensitivity :

<20 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Ficolin-3

Synonyms :

Ficolin-3;Collagen/fibrinogen domain-containing lectin 3 p35;Collagen/fibrinogen domain-containing protein 3;Hakata antigen;FCN3;FCNH, HAKA1;

Protein Name :

Ficolin-3

Molecular Weight :

32903 MW

Protein Function :

May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Has affinity with GalNAc, GlcNAc, D-fucose, as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota.

Subcellular Localization :

Secreted. Found in blood plasma, bronchus, alveolus and bile duct.

Tissue Specificity :

Liver and lung. In liver it is produced by bile duct epithelial cells and hepatocytes. In lung it is produced by both ciliated bronchial epithelial cells and type II alveolar epithelial cells.

Sequence Similarities :

Belongs to the ficolin lectin family.

Background :

Ficolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin (FN1), elastin (ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.

Research Category :

Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Immunology, Secreted Molecules, Signal Transduction

more info or order :

Boster product webpage