Human Ficolin-1 / FCN1 / M Ficolin ELISA Kit PicoKine®

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Ficolin-1 / FCN1 / M Ficolin ELISA Kit PicoKine®

catalog :

EK1257

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 1

Reference
Honore C, Rørvig S, Munthe Fog L, Hummelshøj T, Madsen H, Borregaard N, et al. The innate pattern recognition molecule Ficolin-1 is secreted by monocytes/macrophages and is circulating in human plasma. Mol Immunol. 2008;45:2782-9 pubmed publisher

image

image 1 :

Human Ficolin-1 PicoKine ELISA Kit standard curve

product information

SKU :

EK1257

Product Name :

Human Ficolin-1 / FCN1 / M Ficolin ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

369 USD

Description :

Human Ficolin-1 / FCN1 / M Ficolin ELISA Kit PicoKine® (96 Tests). Quantitate Human FCN1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

FCN1

Uniprot ID :

O00602

Immunogen :

Expression system for standard: NS0; Immunogen sequence: A30-A326

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

0.78 ng/ml - 50 ng/ml

Sensitivity :

<20 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Ficolin-1

Synonyms :

Ficolin-1;Collagen/fibrinogen domain-containing protein 1;Ficolin-A;Ficolin-alpha;M-ficolin;FCN1;FCNM;

Protein Name :

Ficolin-1

Molecular Weight :

35078 MW

Protein Function :

Extracellular lectin functioning as a pattern- recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8 (PubMed:21037097). Binds preferentially to 9-O-acetylated 2-6- linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage.

Subcellular Localization :

Secreted. Cell membrane; Peripheral membrane protein; Extracellular side. Found on the monocyte and granulocyte surface.

Tissue Specificity :

Peripheral blood leukocytes, monocytes and granulocytes. Also detected in spleen, lung, and thymus, may be due to the presence of tissue macrophages or trapped blood in these tissues. Not detected on lymphocytes.

Sequence Similarities :

Belongs to the ficolin lectin family.

Background :

Ficolin-1, and also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. Ficolin 1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.

Research Category :

Immunology, Secreted Molecules

more info or order :

Boster product webpage