Human GPNMB/Osteoactivin ELISA Kit PicoKine® | Boster EK1251 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human GPNMB/Osteoactivin ELISA Kit PicoKine®

catalog :

EK1251

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 1

Reference
Li Y, Zhang L, Li X, Cui D, Zheng H, Yang S, et al. Glycoprotein nonmetastatic B as a prognostic indicator in small cell lung cancer. APMIS. 2014;122:140-6 pubmed publisher

image

image 1 :

Human GPNMB/Osteoactivin PicoKine ELISA Kit standard curve

product information

SKU :

EK1251

Product Name :

Human GPNMB/Osteoactivin ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

369 USD

Description :

Human GPNMB/Osteoactivin ELISA Kit PicoKine® (96 Tests). Quantitate Human GPNMB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

GPNMB

Uniprot ID :

Q14956

Immunogen :

Expression system for standard: NS0; Immunogen sequence: K23-P486

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

polyclonal antibody from goat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Transmembrane glycoprotein NMB

Synonyms :

Transmembrane glycoprotein NMB;Transmembrane glycoprotein HGFIN;GPNMB;HGFIN, NMB;UNQ1725/PRO9925;

Protein Name :

Transmembrane glycoprotein NMB

Molecular Weight :

63923 MW

Protein Function :

Could be a melanogenic enzyme.

Subcellular Localization :

Cell membrane; Single-pass type I membrane protein. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Detected at the cell surface in different types of cancers cells, including glioblastoma multiforme cells and most melanoma cell lines.

Tissue Specificity :

Up-regulated in various cancer cells, including in glioblastoma multiforme. Expressed in many melanoma cells, as well as in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level). Hardly detectable in healthy brain.

Sequence Similarities :

Belongs to the PMEL/NMB family.

Background :

Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.

Research Category :

Cancer, Immunology, Innate Immunity, Macrophage / Inflamm., Tumor Immunology, Tumor-Associated Antigens

more info or order :

Boster product webpage