Rat MMP-8/Neutrophil collagenase ELISA Kit PicoKine® | Boster EK1248 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Rat MMP-8/Neutrophil collagenase ELISA Kit PicoKine®

catalog :

EK1248

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1248

Product Name :

Rat MMP-8/Neutrophil collagenase ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Rat MMP-8 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Mmp8 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Reactivity :

Rat

Application :

ELISA

Gene Name :

Mmp8

Uniprot ID :

O88766

Immunogen :

Expression system for standard: NS0; Immunogen sequence: L21-P466

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Neutrophil collagenase

Synonyms :

Neutrophil collagenase;3.4.24.34;Matrix metalloproteinase-8;MMP-8;Mmp8;

Protein Name :

Neutrophil collagenase

Molecular Weight :

53277 MW

Protein Function :

Can degrade fibrillar type I, II, and III collagens.

Subcellular Localization :

Cytoplasmic granule. Secreted, extracellular space, extracellular matrix . Stored in intracellular granules.

Tissue Specificity :

Can degrade fibrillar type I, II, and III collagens.

Sequence Similarities :

Belongs to the peptidase M10A family.

Background :

Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

Research Category :

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton / Ecm, Ecm Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis / Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tumor Biomarkers, Vascular Inflammation