Human Midkine ELISA Kit PicoKine® | Boster EK1235 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Midkine ELISA Kit PicoKine®

catalog :

EK1235

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 1

Reference
Shaygannejad V, Montazeri S, Jamshidian A, Tahani S, Gharagozloo M, Ashtari F, et al. Correlation of midkine serum level with pro- and anti-inflamatory cytokines in multiple sclerosis. Iran J Immunol. 2014;11:134-8 pubmed publisher

image

image 1 :

Human Midkine PicoKine ELISA Kit standard curve

product information

SKU :

EK1235

Product Name :

Human Midkine ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

369 USD

Description :

Human Midkine ELISA Kit PicoKine® (96 Tests). Quantitate Human MDK in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

MDK

Uniprot ID :

P21741

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: K23-D143

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

78 pg/ml - 5,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Midkine

Synonyms :

Midkine;MK;Amphiregulin-associated protein;ARAP;Midgestation and kidney protein;Neurite outgrowth-promoting factor 2;Neurite outgrowth-promoting protein;MDK;MK1, NEGF2;

Protein Name :

Midkine

Molecular Weight :

15585 MW

Protein Function :

Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (By similarity).

Subcellular Localization :

Secreted.

Tissue Specificity :

Expressed in various tumor cell lines. In insulinoma tissue predominantly expressed in precancerous lesions.

Background :

Midkine (MK or MDK) also known as neurite growth-promoting factor 2 (NEGF2) is a protein that in humans is encoded by the MDK gene. Midkine is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin (NEGF1, 46% homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. MK is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. A molecular complex containing receptor-type tyrosine phosphatase zeta (PTPζ), low density lipoprotein receptor-related protein (LRP1), anaplastic leukemia kinase (ALK) and syndecans is considered to be its receptor.

Research Category :

Growth And Development, Neurogenesis, Neurology Process, Neuroscience

more info or order :

Boster product webpage