Mouse CD13/Aminopeptidase N ELISA Kit PicoKine® | Boster EK1222 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse CD13/Aminopeptidase N ELISA Kit PicoKine®

catalog :

EK1222

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1222

Product Name :

Mouse CD13/Aminopeptidase N ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Mouse CD13/Aminopeptidase N ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Anpep in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

ANPEP

Uniprot ID :

P97449

Immunogen :

Expression system for standard: NS0; Immunogen sequence: K69-S966

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

312 pg/ml - 20,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Aminopeptidase N

Synonyms :

Aminopeptidase N;AP-N;mAPN;3.4.11.2;Alanyl aminopeptidase;Aminopeptidase M;AP-M;Membrane protein p161;Microsomal aminopeptidase;CD13;Anpep;Lap-1, Lap1;

Protein Name :

Aminopeptidase N

Molecular Weight :

109651 MW

Protein Function :

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May have a role in angiogenesis (By similarity). Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells.

Subcellular Localization :

Membrane; Single-pass type II membrane protein.

Tissue Specificity :

Highly expressed in intestinal tract and kidney, present in liver, lymph node, spleen, and brain. Found as well in monocytes, macrophages, dendritic cells, veiled cells and B-cells but not on T-cells and thymocytes.

Background :

Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.

Research Category :

Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Mesenchymal Stem Cells, Myeloid, Stem Cells, Surface Molecules