Human SPARC ELISA Kit PicoKine® | Boster EK1210 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human SPARC ELISA Kit PicoKine®

catalog :

EK1210

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 1

Reference
Ikuta Y, Nakatsura T, Kageshita T, Fukushima S, Ito S, Wakamatsu K, et al. Highly sensitive detection of melanoma at an early stage based on the increased serum secreted protein acidic and rich in cysteine and glypican-3 levels. Clin Cancer Res. 2005;11:8079-88 pubmed

image

image 1 :

Human SPARC PicoKine ELISA Kit standard curve

product information

SKU :

EK1210

Product Name :

Human SPARC ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

369 USD

Description :

Human SPARC ELISA Kit PicoKine® (96 Tests). Quantitate Human SPARC in cell culture supernatants, serum and human milk. Sensitivity: 20pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

SPARC

Uniprot ID :

P09486

Immunogen :

Expression system for standard: NS0; Immunogen sequence: A18-I303

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

0.78 ng/ml - 50 ng/ml

Sensitivity :

<20 pg/ml

Sample Type :

cell culture supernatants, serum and human milk

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

SPARC

Synonyms :

SPARC;Basement-membrane protein 40;BM-40;Osteonectin;ON;Secreted protein acidic and rich in cysteine;SPARC;ON;

Protein Name :

SPARC

Molecular Weight :

34632 MW

Protein Function :

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca (2+) with a low affinity and an EF-hand loop that binds a Ca (2+) ion with a high affinity.

Subcellular Localization :

Secreted, extracellular space, extracellular matrix, basement membrane . In or around the basement membrane.

Sequence Similarities :

Belongs to the SPARC family.

Background :

SPARC (secreted protein acidic and rich in cysteine), also known as Osteonectin, is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found.

Research Category :

Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Cytoskeleton / Ecm, Developmental Biology, Extracellular Matrix, Lineage Markers, Lineage Specification, Mesenchymal Stem Cells, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Stem Cells, Structures, Tyrosine Kinases

more info or order :

Boster product webpage