Human WISP1/CCN4 ELISA Kit PicoKine® | Boster EK1204 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human WISP1/CCN4 ELISA Kit PicoKine®

catalog :

EK1204

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1204

Product Name :

Human WISP1/CCN4 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human WISP1/CCN4 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCN4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

WISP1

Uniprot ID :

O95388

Immunogen :

Expression system for standard: NS0; Immunogen sequence: T23-N367

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

31.2 pg/ml - 2,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Synonyms :

WNT1-inducible-signaling pathway protein 1;WISP-1;CCN family member 4;Wnt-1-induced secreted protein;WISP1;CCN4;

Molecular Weight :

40331 MW

Protein Function :

Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X (L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan.

Subcellular Localization :

Secreted.

Tissue Specificity :

Expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Isoform 2 is expressed predominantly in scirrhous gastric carcinoma and, weakly in placenta. Overexpression is associated with several cancers including breast cancer and colon tumors. Isoform 2 is overexpressed in scirrhous gastric carcinoma.

Sequence Similarities :

Belongs to the CCN family.

Background :

WISP1 (WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.

Research Category :

Epigenetics and Nuclear Signaling, Transcription, DNA / RNA, DNA Damage & Repair, DNA Damage Response, Signaling Pathways, Stem Cells, Domain Families, Secreted, Developmental Families