Mouse MFGE8 / Lactadherin / Milk Fat Globule 1 ELISA Kit PicoKine®

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse MFGE8 / Lactadherin / Milk Fat Globule 1 ELISA Kit PicoKine®

catalog :

EK1194

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1194

Product Name :

Mouse MFGE8 / Lactadherin / Milk Fat Globule 1 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Mouse MFGE8 / Lactadherin / Milk Fat Globule 1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Mfge8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

MFGE8

Uniprot ID :

P21956

Immunogen :

Expression system for standard: NS0; Immunogen sequence: A23-C463

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

62.5 pg/ml - 4,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Lactadherin

Synonyms :

Lactadherin;MFGM;Milk fat globule-EGF factor 8;MFG-E8;SED1;Sperm surface protein SP47;MP47;Mfge8;

Protein Name :

Lactadherin

Molecular Weight :

51241 MW

Protein Function :

Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha- v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction (By similarity). Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization.

Subcellular Localization :

Membrane ; Peripheral membrane protein . Secreted .

Tissue Specificity :

Mammary epithelial cell surfaces and spermatozoan. Isoform 2 is present in brain, heart, kidney and spleen and at low levels in lung, liver, small intestine and testis.

Sequence Similarities :

Contains 2 EGF-like domains.

Background :

MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.

Research Category :

Angiogenesis, Apoptosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Metabolism, Metabolism Processes, Pathways And Processes