Human Kininogen-1 / KNG1 / HMW Kininogen ELISA Kit PicoKine®

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Kininogen-1 / KNG1 / HMW Kininogen ELISA Kit PicoKine®

catalog :

EK1154

quantity :

96 wells/kit, with removable strips.

price :

399 USD

more info or order :

Boster product webpage

citations: 1

Reference
Wang J, Wang X, Lin S, Chen C, Wang C, Ma Q, et al. Identification of kininogen-1 as a serum biomarker for the early detection of advanced colorectal adenoma and colorectal cancer. PLoS ONE. 2013;8:e70519 pubmed publisher

image

image 1 :

Human Kininogen-1/KNG1 ELISA Kit standard curve

product information

SKU :

EK1154

Product Name :

Human Kininogen-1 / KNG1 / HMW Kininogen ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

399 USD

Description :

Human Kininogen-1 / KNG1 / HMW Kininogen ELISA Kit PicoKine® (96 Tests). Quantitate Human KNG1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

KNG1

Uniprot ID :

P01042

Immunogen :

Expression system for standard: NS0; Immunogen sequence: Q19-S644

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<20 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Kininogen-1

Synonyms :

Kininogen-1;Alpha-2-thiol proteinase inhibitor;Fitzgerald factor;High molecular weight kininogen;HMWK;Williams-Fitzgerald-Flaujeac factor;Kininogen-1 heavy chain;T-kinin;Ile-Ser-Bradykinin;Bradykinin;Kallidin I;Lysyl-bradykinin;Kallidin II;Kininogen-1 light chain;Low molecular weight growth-promoting factor;KNG1;BDK, KNG;

Protein Name :

Kininogen-1

Molecular Weight :

71957 MW

Protein Function :

(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting.

Subcellular Localization :

Secreted, extracellular space.

Tissue Specificity :

Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.

Sequence Similarities :

Contains 3 cystatin kininogen-type domains.

Background :

Kininogen-1 (KNG1), also known as alpha-2-thiol proteinase inhibitor, Williams-Fitzgerald-Flaujeac factor or the HMWK-kallikrein factor, is a protein that in humans is encoded by the KNG1 gene. It is mapped to 3q27.3. The KNG1 gene uses alternative splicing to generate two different proteins – high – molecular - weight kininogen (HMWK) and low - molecular- weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. In addition to that, KNG1 is a constituent of the blood coagulation system as well as the kinin-kallikrein system.

Research Category :

Cardiovascular, Cytoskeleton / Ecm, Ecm Enzymes, Extracellular Matrix, Signal Transduction, Vasculature

more info or order :

Boster product webpage