Human CCL14/HCC-1 ELISA Kit PicoKine® | Boster EK1123 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human CCL14/HCC-1 ELISA Kit PicoKine®

catalog :

EK1123

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1123

Product Name :

Human CCL14/HCC-1 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human CCL14/HCC-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

CCL14

Uniprot ID :

Q16627

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: T20-N93

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

C-C motif chemokine 14

Synonyms :

C-C motif chemokine 14;Chemokine CC-1/CC-3;HCC-1/HCC-3;HCC-1 (1-74);NCC-2;Small-inducible cytokine A14;HCC-1 (3-74);HCC-1 (4-74);HCC-1 (9-74);CCL14;NCC2, SCYA14;

Protein Name :

C-C motif chemokine 14

Molecular Weight :

10678 MW

Protein Function :

Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca (2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1 (9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.

Subcellular Localization :

Secreted.

Tissue Specificity :

Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma.

Sequence Similarities :

Belongs to the intercrine beta (chemokine CC) family.

Background :

Chemokine (C-C motif) ligand 14 (CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.

Research Category :

Chemokines, Cofactors, Vitamins / Minerals, Immunology, Innate Immunity, Metabolism, Pathways And Processes, Signal Transduction, Vitamins / Minerals