Mouse Thrombomodulin ELISA Kit PicoKine® | Boster EK1119 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse Thrombomodulin ELISA Kit PicoKine®

catalog :

EK1119

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1119

Product Name :

Mouse Thrombomodulin ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Mouse Thrombomodulin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Thbd in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

THBD

Uniprot ID :

P15306

Immunogen :

Expression system for standard: NS0; Immunogen sequence: L17-S517

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

62.5 pg/ml - 4,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Thrombomodulin

Synonyms :

Thrombomodulin;TM;Fetomodulin;CD141;Thbd;

Protein Name :

Thrombomodulin

Molecular Weight :

61868 MW

Protein Function :

Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

Subcellular Localization :

Membrane; Single-pass type I membrane protein.

Tissue Specificity :

Endothelial cells are unique in synthesizing thrombomodulin.

Sequence Similarities :

Contains 1 C-type lectin domain.

Background :

Thrombomodulin (TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor (TAFI,aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene (THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome (aHUS).

Research Category :

Cardiovascular, Stem Cells