Human A2M/Alpha 2-Macroglobulin ELISA Kit PicoKine® | Boster EK1118 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human A2M/Alpha 2-Macroglobulin ELISA Kit PicoKine®

catalog :

EK1118

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1118

Product Name :

Human A2M/Alpha 2-Macroglobulin ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human A2M/Alpha 2-Macroglobulin ELISA Kit PicoKine® (96 Tests). Quantitate Human A2M in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

A2M

Uniprot ID :

P01023

Immunogen :

Expression system for standard: from human plasma

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

625 pg/ml - 40,000 pg/ml

Sensitivity :

<20 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

polyclonal antibody from goat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Alpha-2-macroglobulin

Synonyms :

Alpha-2-macroglobulin;Alpha-2-M;C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5;A2M;CPAMD5;FWP007;

Protein Name :

Alpha-2-macroglobulin

Molecular Weight :

163291 MW

Protein Function :

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Subcellular Localization :

Secreted .

Tissue Specificity :

Secreted in plasma.

Sequence Similarities :

Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.

Background :

Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Research Category :

Cell Biology, Complement, Immunology, Innate Immunity, Protease Inhibitors, Proteolysis / Ubiquitin