Mouse Granzyme B ELISA Kit PicoKine® | Boster EK1115 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse Granzyme B ELISA Kit PicoKine®

catalog :

EK1115

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1115

Product Name :

Mouse Granzyme B ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Mouse Granzyme B ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Gzmb in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

Gzmb

Uniprot ID :

P04187

Immunogen :

Expression system for standard: NS0; Immunogen sequence: I21-S247

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

polyclonal antibody from goat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Granzyme B(G,H)

Synonyms :

Granzyme B (G,H);3.4.21.79;CTLA-1;Cytotoxic cell protease 1;CCP1;Fragmentin-2;Gzmb;Ctla-1, Ctla1;

Protein Name :

Granzyme B(G,H)

Molecular Weight :

27470 MW

Protein Function :

This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis (By similarity).

Subcellular Localization :

Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.

Background :

Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.

Research Category :

Adaptive Immunity, Apoptosis, Cell Biology, Immunology, Innate Immunity, Nk Cells, T Cells