Human Granzyme B ELISA Kit PicoKine® | Boster EK1114 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Granzyme B ELISA Kit PicoKine®

catalog :

EK1114

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 1

Reference
Goldbach Mansky R, Suson S, Wesley R, Hack C, El Gabalawy H, Tak P. Raised granzyme B levels are associated with erosions in patients with early rheumatoid factor positive rheumatoid arthritis. Ann Rheum Dis. 2005;64:715-21 pubmed

image

image 1 :

Human Granzyme B PicoKine ELISA Kit standard curve

product information

SKU :

EK1114

Product Name :

Human Granzyme B ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

369 USD

Description :

Human Granzyme B ELISA Kit PicoKine® (96 Tests). Quantitate Human GZMB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

GZMB

Uniprot ID :

P10144

Immunogen :

Expression system for standard: NS0; Immunogen sequence: I21-Y247

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Granzyme B

Synonyms :

Granzyme B;3.4.21.79;C11;CTLA-1;Cathepsin G-like 1;CTSGL1;Cytotoxic T-lymphocyte proteinase 2;Lymphocyte protease;Fragmentin-2;Granzyme-2;Human lymphocyte protein;HLP;SECT;T-cell serine protease 1-3E;GZMB;CGL1, CSPB, CTLA1, GRB;

Protein Name :

Granzyme B

Molecular Weight :

27716 MW

Protein Function :

This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Subcellular Localization :

Cytoplasmic granule . Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.

Sequence Similarities :

Belongs to the peptidase S1 family. Granzyme subfamily.

Background :

Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.

Research Category :

Adaptive Immunity, Apoptosis, Cell Biology, Immunology, Innate Immunity, Nk Cells, T Cells

more info or order :

Boster product webpage