Human ACE2 PicoKine ELISA Kit | Boster EK0997 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human ACE2 PicoKine ELISA Kit

catalog :

EK0997

quantity :

96 wells/kit, with removable strips.

citations: 1

Reference
Matsushita T, Isobe N, Kawajiri M, Mogi M, Tsukuda K, Horiuchi M, et al. CSF angiotensin II and angiotensin-converting enzyme levels in anti-aquaporin-4 autoimmunity. J Neurol Sci. 2010;295:41-5 pubmed publisher

product information

sku :

EK0997

status :

Enabled

name :

Human ACE2 PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

ACE2

assay range :

62.5pg/ml-4000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human ACE2 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. ACE2 tissue specificity: Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ACE2. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

Q9BYF1

host :

immunogen :

Expression system for standard: NSO; Immunogen sequence: Q18-S740

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma(heparin).

applications :

ELISA

reactivity :

Human

image labels :

Human ACE2 PicoKine ELISA Kit standard curve

anticoagulant :

heparin

assay procedure step 1 :

4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml

background :

AngiotensinI-converting enzyme 2(ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.

competitor equivalent skus :

sc 73668 sc 21834 sc 17719 sc 20998 sc 17720 sc 390851 sc 17719 R

research category :

Cardiovascular, Cell Biology, Contractility, Metalloprotease, Proteolysis / Ubiquitin, Proteolytic Enzymes, Vasculature

synonyms :

Angiotensin-converting enzyme 2;3.4.17.23;ACE-related carboxypeptidase;Angiotensin-converting enzyme homolog;ACEH;Metalloprotease MPROT15;Processed angiotensin-converting enzyme 2;ACE2;UNQ868/PRO1885;

gene full name :

Angiotensin-converting enzyme 2

molecular weight :

92463 MW

protein function :

Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.

subcellular localization :

Processed angiotensin-converting enzyme 2: Secreted.

tissue specificity :

Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.

protein name :

Angiotensin-converting enzyme 2

sequence similarities :

Belongs to the peptidase M2 family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 163 424 1746 176 432 1911 Standard deviation 7 16.96 114.33 9.32 18.14 144.33 CV(%) 4.3% 4% 6.6% 5.3% 4.2% 7.6%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 163 147 171 161 160 8.64 5.4% Sample 2 424 478 483 434 454 26.05 5.7% Sample 3 1746 1780 1478 1557 1640 126.46 7.7% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.162 0.227 0.281 0.402 0.680 0.972 1.658 2.214

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53