Human ALCAM/CD166 ELISA Kit PicoKine® | Boster EK0995 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human ALCAM/CD166 ELISA Kit PicoKine®

catalog :

EK0995

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 1

Reference
Ihnen M, Kress K, Kersten J, Kilic E, Choschzick M, Zander H, et al. Relevance of activated leukocyte cell adhesion molecule (ALCAM) in tumor tissue and sera of cervical cancer patients. BMC Cancer. 2012;12:140 pubmed publisher

image

image 1 :

Human ALCAM PicoKine ELISA Kit standard curve

product information

SKU :

EK0995

Product Name :

Human ALCAM/CD166 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

369 USD

Description :

Human ALCAM/Cd166 ELISA Kit PicoKine® (96 Tests). Quantitate Human ALCAM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

ALCAM

Uniprot ID :

Q13740

Immunogen :

Expression system for standard: NS0; Immunogen sequence: W28-A526

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

312 pg/ml - 20,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

CD166 antigen

Synonyms :

CD166 antigen;Activated leukocyte cell adhesion molecule;CD166;ALCAM;MEMD;

Protein Name :

CD166 antigen

Molecular Weight :

65102 MW

Protein Function :

Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.

Subcellular Localization :

Membrane; Single-pass type I membrane protein.

Tissue Specificity :

Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines.

Sequence Similarities :

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

Background :

CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

Research Category :

Adaptive Immunity, B Cells, Immunology, T Cells

more info or order :

Boster product webpage