Mouse Complement C5a ELISA Kit PicoKine® | Boster EK0987 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse Complement C5a ELISA Kit PicoKine®

catalog :

EK0987

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 1

Reference
Munenaga S, Ouhara K, Hamamoto Y, Kajiya M, Takeda K, Yamasaki S, et al. The involvement of C5a in the progression of experimental arthritis with Porphyromonas gingivalis infection in SKG mice. Arthritis Res Ther. 2018;20:247 pubmed publisher

image

image 1 :

Mouse Complement C5a PicoKine ELISA Kit standard curve

product information

SKU :

EK0987

Product Name :

Mouse Complement C5a ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

369 USD

Description :

Mouse Complement C5a ELISA Kit PicoKine® (96 Tests). Quantitate Mouse C5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

Uniprot ID :

P06684

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: N679-R755

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Complement C5

Synonyms :

Complement C5;Hemolytic complement;Complement C5 beta chain;Complement C5 alpha chain;C5a anaphylatoxin;Complement C5 alpha' chain;C5;Hc;

Protein Name :

Complement C5

Molecular Weight :

188878 MW

Protein Function :

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.

Subcellular Localization :

Secreted.

Sequence Similarities :

Contains 1 anaphylatoxin-like domain.

Background :

C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.

Research Category :

ChIP’ing Antibodies, Domain Families, Epigenetics and Nuclear Signaling, Hlh / Leucine Zipper, Immunology, Innate Immunity, Transcription

more info or order :

Boster product webpage