Mouse Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine® | Boster EK0975 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine®

catalog :

EK0975

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK0975

Product Name :

Mouse Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Mouse Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Chi3l1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

CHI3L1

Uniprot ID :

Q61362

Immunogen :

Expression system for standard: NS0; Immunogen sequence: Y22-A381

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

62.5 pg/ml - 4,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Chitinase-3-like protein 1

Synonyms :

Chitinase-3-like protein 1;BRP39 protein;Breast regression protein 39;Cartilage glycoprotein 39;CGP-39;GP-39;Chi3l1;Brp39, Chil1;

Protein Name :

Chitinase-3-like protein 1

Molecular Weight :

43893 MW

Protein Function :

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.

Subcellular Localization :

Secreted, extracellular space. Cytoplasm. Endoplasmic reticulum. Detected in bronchoalveolar lavage (BAL) fluids. Localizes mainly to endoplasmic reticulum when overexpressed in cells, with some protein also detected throughout the cytoplasm.

Tissue Specificity :

Detected in lung in pulmonary macrophages and alveolar type 2 cells and in bronchoalveolar lavage (BAL) fluids. Expressed in mammary tumor cells (at protein level). Expressed in lung. Not detected in non-inflammatory colon.

Sequence Similarities :

Belongs to the glycosyl hydrolase 18 family.

Background :

Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.

Research Category :

Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Signal Transduction