Human MMP-14 PicoKine ELISA Kit | Boster EK0951 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human MMP-14 PicoKine ELISA Kit

catalog :

EK0951

quantity :

96 wells/kit, with removable strips.

product information

sku :

EK0951

status :

Enabled

name :

Human MMP-14 PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

MMP14

assay range :

156pg/ml-10000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human MMP14 with <15pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. MMP14 tissue specificity: Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP-14. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P50281

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: A21-S538

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<15pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma(heparin).

applications :

ELISA

reactivity :

Human

image labels :

Human MMP-14 PicoKine ELISA Kit standard curve

anticoagulant :

heparin

assay procedure step 1 :

10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml

background :

Matrix metalloproteinase-14 is an enzyme that in humans is encoded by the MMP14 gene. It is mapped to chromosome 14q11-q12. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive pro-proteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted.

research category :

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cell Biology, Cytoskeleton / Ecm, Ecm Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metabolism, Metabolism Processes, Metalloprotease, Pathways And Processes, Proteolysis / Ubiquitin, Proteolytic Enzymes, Response To Hypoxia, Signal Transduction, Tumor Biomarkers

synonyms :

Matrix metalloproteinase-14;MMP-14;3.4.24.80;MMP-X1;Membrane-type matrix metalloproteinase 1;MT-MMP 1;MTMMP1;Membrane-type-1 matrix metalloproteinase;MT1-MMP;MT1MMP;MMP14;

gene full name :

Matrix metalloproteinase-14

molecular weight :

65894 MW

protein function :

Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro- MMP2.

subcellular localization :

Membrane ; Single-pass type I membrane protein . Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm.

tissue specificity :

Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.

protein name :

Matrix metalloproteinase-14

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 218 1813 5699 196 1642 5266 Standard deviation 8.93 90.65 302.04 8.62 94.13 321.22 CV(%) 4.1% 5% 5.3% 4.4% 5.8% 6.1%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 218 218 198 218 213 8.66 4% Sample 2 1813 2134 1935 1919 1950 115.98 5.9% Sample 3 5699 5486 4819 4823 5206 393.03 7.5% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.073 0.096 0.145 0.258 0.541 1.086 2.049

tmb incubation time :

15-25min

principle :

a monoclonal antibody from mouse a biotinylated polyclonal antibody from goat

last modified :

5/7/19 5:40