Human MMP-12 ELISA Kit PicoKine® | Boster EK0950 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human MMP-12 ELISA Kit PicoKine®

catalog :

EK0950

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 1

Reference
Demedts I, Morel Montero A, Lebecque S, Pacheco Y, Cataldo D, Joos G, et al. Elevated MMP-12 protein levels in induced sputum from patients with COPD. Thorax. 2006;61:196-201 pubmed

image

image 1 :

Human MMP-12 PicoKine ELISA Kit standard curve

product information

SKU :

EK0950

Product Name :

Human MMP-12 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

369 USD

Description :

Human MMP-12 ELISA Kit PicoKine® (96 Tests). Quantitate Human MMP12 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

MMP12

Uniprot ID :

P39900

Immunogen :

Expression system for standard: NS0; Immunogen sequence: L17-C470

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

62.5 pg/ml - 4,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin)

Capture/Detection Antibodies :

polyclonal antibody from goat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Macrophage metalloelastase

Synonyms :

Macrophage metalloelastase;MME;3.4.24.65;Macrophage elastase;ME;hME;Matrix metalloproteinase-12;MMP-12;MMP12;HME;

Protein Name :

Macrophage metalloelastase

Molecular Weight :

54002 MW

Protein Function :

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.

Subcellular Localization :

Secreted, extracellular space, extracellular matrix .

Tissue Specificity :

Found in alveolar macrophages but not in peripheral blood monocytes.

Sequence Similarities :

Belongs to the peptidase M10A family.

Background :

Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.

Research Category :

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cytoskeleton / Ecm, Ecm Enzymes, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Tumor Biomarkers

more info or order :

Boster product webpage