Mouse IL-12(p40) ELISA Kit PicoKine® | Boster EK0932 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse IL-12(p40) ELISA Kit PicoKine®

catalog :

EK0932

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 1

Reference
Nigg A, Zahn S, Rückerl D, Holscher C, Yoshimoto T, Ehrchen J, et al. Dendritic cell-derived IL-12p40 homodimer contributes to susceptibility in cutaneous leishmaniasis in BALB/c mice. J Immunol. 2007;178:7251-8 pubmed

image

image 1 :

Mouse IL-12(p40) PicoKine ELISA Kit standard curve

product information

SKU :

EK0932

Product Name :

Mouse IL-12(p40) ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

369 USD

Description :

Mouse IL-12 (p40) ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il12b in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

IL12B

Uniprot ID :

P43432

Immunogen :

Expression system for standard: NS0; Immunogen sequence: M23-S335

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Interleukin-12 subunit beta

Synonyms :

Interleukin-12 subunit beta;IL-12B;Cytotoxic lymphocyte maturation factor 40 kDa subunit;CLMF p40;IL-12 subunit p40;Il12b;

Protein Name :

Interleukin-12 subunit beta

Molecular Weight :

38235 MW

Protein Function :

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Subcellular Localization :

Secreted.

Sequence Similarities :

Belongs to the type I cytokine receptor family. Type 3 subfamily.

Background :

Interleukin-12 (IL12, formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40-kDa subunit (p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit (p35) is related to IL6 and GCSF.2 Both IL-12 p40 (-/-) and p35 (-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin (IL)-12 has been cloned on the basis of its ability to activate natural killer (NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon (IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.

Research Category :

Macrophage / Inflamm., Interleukins

more info or order :

Boster product webpage