Human IL-33 ELISA Kit PicoKine® | Boster EK0929 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-33 ELISA Kit PicoKine®

catalog :

EK0929

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 1

Reference
Zheng L, Wang W, Ni J, Li Z, Xiao T, Zhang Q, et al. Plasma interleukin 33 level in patients with osteonecrosis of femoral head: an alarmin for osteonecrosis of the femoral head?. J Investig Med. 2014;62:635-7 pubmed publisher

image

image 1 :

Human IL-33 PicoKine ELISA Kit standard curve

product information

SKU :

EK0929

Product Name :

Human IL-33 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

369 USD

Description :

Human IL-33 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL33 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

IL33

Uniprot ID :

O95760

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: S112-T270

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysate

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Interleukin-33

Synonyms :

Interleukin-33;IL-33;Interleukin-1 family member 11;IL-1F11;Nuclear factor from high endothelial venules;NF-HEV;Interleukin-33 (95-270);Interleukin-33 (99-270);Interleukin-33 (109-270);IL33;C9orf26, IL1F11, NFHEV;

Protein Name :

Interleukin-33

Molecular Weight :

30759 MW

Protein Function :

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).

Subcellular Localization :

Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes. Translocation from the nucleus occurs upon biomechanical strain, depends on an intact microtubule network, and is ATP-dependent.

Tissue Specificity :

Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.

Background :

Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. The IL33 gene maps to chromosome 9p24.1 by using of genomic database analysis. Recombinant mature human IL33 bound to ST2. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell (CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.

Research Category :

Angiogenesis, Cardiovascular, Cytokines, Hypertrophy, Immunology, Innate Immunity, Interleukins

more info or order :

Boster product webpage