Mouse Clusterin / Apolipoprotein J ELISA Kit PicoKine®

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse Clusterin / Apolipoprotein J ELISA Kit PicoKine®

catalog :

EK0923

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK0923

Product Name :

Mouse Clusterin / Apolipoprotein J ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Mouse Clusterin / Apolipoprotein J ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Clu in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

CLU

Uniprot ID :

Q06890

Immunogen :

Expression system for standard: NS0; Immunogen sequence: D22-R226 (beta) & S227-E448 (alpha)

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

312 pg/ml - 20,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Clusterin

Synonyms :

Clusterin;Apolipoprotein J;Apo-J;Clustrin;Sulfated glycoprotein 2;SGP-2;Clusterin beta chain;Clusterin alpha chain;Clu;Apoj, Msgp-2;

Protein Name :

Clusterin

Molecular Weight :

51656 MW

Protein Function :

Functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Promotes apoptosis when in the nucleus. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation (By similarity).

Subcellular Localization :

Secreted . Nucleus . Cytoplasm . Mitochondrion membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytosol . Microsome . Endoplasmic reticulum . Cytoplasmic vesicle, secretory vesicle, chromaffin granule . Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis (By similarity).

Tissue Specificity :

Most abundant in stomach, liver, brain, and testis, with intermediate levels in heart, ovary and kidney.

Background :

Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.

Research Category :

Cancer, Cardiovascular, Chaperones, Complement, Immunology, Innate Immunity, Lipid Metabolism, Lipids / Lipoproteins, Metabolism, Protein Trafficking, Signal Transduction, Tumor Biomarkers