Human Endostatin ELISA Kit PicoKine¬Æ | Boster EK0886 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Endostatin ELISA Kit PicoKine¬Æ

catalog :

EK0886

more info or order :

Boster product webpage

citations: 4

Reference
Salza R, Oudart J, Ramont L, Maquart F, Bakchine S, Thoannes H, et al. Endostatin level in cerebrospinal fluid of patients with Alzheimer's disease. J Alzheimers Dis. 2015;44:1253-61 pubmed publisher
Ã…kerfeldt T, Gunningberg L, Swenne C, Ronquist G, Larsson A. Elective orthopedic and cardiopulmonary bypass surgery causes a reduction in serum endostatin levels. Eur J Med Res. 2014;19:61 pubmed publisher
Hu M, Hu Y, Zhang H, Jia W, Qian Z, Yang Y, et al. Clinical significance of serum and tumor tissue endostatin evaluation in operable non-small cell lung cancer. Biomed Rep. 2014;2:898-904 pubmed
Liu Y, Zhou Q, Lu Y, Zhu W, Wang Y, Zhang J, et al. [Serum levels of endostatin and VEGF in non-small cell lung cancer patients and the relationship with the clinical pathophysiological characteristics]. Zhongguo Fei Ai Za Zhi. 2004;7:50-4 pubmed publisher

image

image 1 :

Human Endostatin PicoKine ELISA Kit standard curve

product information

sku :

EK0886

status :

name :

Human Endostatin ELISA Kit PicoKine¬Æ

url key :

human-endostatin-picokine-trade-elisa-kit-ek0886-boster

product category :

Picokine ELISA Kits

gene name :

COL18A1

price :

369

assay range :

156 pg/ml - 10,000 pg/ml

description :

Human Endostatin ELISA Kit PicoKine (96 Tests). Quantitate Human COL18A1 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml.

short description :

Human Endostatin ELISA Kit PicoKine (96 Tests). Quantitate Human COL18A1 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P39060

immunogen :

Expression system for standard: E.coli; Immunogen sequence: H1154-S1335

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

20 ng/tube

sample type :

cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

application details :

Dilution ratio of 1:20, concentration in serum is 30-50ng/ml

applications :

ELISA

reactivity :

Human

images :

/antibody/ek0886-2-ELISA-human-endostatin-picokine-elisa-kit.jpg

image labels :

Human Endostatin PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.294 0.596 0.980 1.323 1.555 1.695 1.916

created at :

7/8/14 9:17

updated at :

3/2/22 2:54

meta title :

Human Endostatin ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human Endostatin ELISA Kit, Alpha 1 type XVIII collagen

meta description :

Human Endostatin ELISA Kit PicoKine (96 Tests). Quantitate Human COL18A1 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml.

rating value :

97.6

anticoagulant :

heparin or EDTA

assay procedure step 1 :

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

background :

Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And it is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.

competitor equivalent skus :

sc 16651 sc 25720 sc 32720 sc 514355

research category :

Angiogenesis,Cancer,Cardiovascular,Collagen,Cytoskeleton / Ecm,Ecm Proteins,Extracellular Matrix,Invasion/Microenvironment,Signal Transduction

synonyms :

Collagen alpha-1 (XVIII) chain;Endostatin;COL18A1;

gene full name :

Collagen alpha-1(XVIII) chain

molecular weight :

178188 MW

protein function :

COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.

subcellular localization :

Secreted, extracellular space, extracellular matrix .

tissue specificity :

Present in multiple organs with highest levels in liver, lung and kidney.

protein name :

Collagen alpha-1(XVIII) chain

sequence similarities :

Belongs to the multiplexin collagen family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1523 5144 251 1462 5243 Standard deviation 14.07 109.65 216.04 14.3 130.11 319.82 CV (%) 5.7% 7.2% 4.2% 5.7% 8.9% 6.1%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 238 232 204 230 16.06 6.9% Sample 2 1523 1309 1362 1479 1418 86.25 6% Sample 3 5144 5141 5851 5778 5478 336.99 6.1% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

25408220, 25380724, 25279166, 21211193

template :

elisa-kits

more info or order :

Boster product webpage