Rat KIM1 ELISA Kit PicoKine¬Æ | Boster EK0882 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Rat KIM1 ELISA Kit PicoKine¬Æ

catalog :

EK0882

more info or order :

Boster product webpage

citations: 1

Reference
Ji X, Ma L, Lu J, Zhang S, Huang Y, Hou X, et al. [Expression of kidney injury molecule-1 in renal ischemic postconditioning and its protective effect against renal ischemia-reperfusion injury in rats]. Beijing Da Xue Xue Bao Yi Xue Ban. 2012;44:511-7 pubmed

image

image 1 :

Rat KIM1 PicoKine ELISA Kit standard curve

product information

sku :

EK0882

status :

name :

Rat KIM1 ELISA Kit PicoKine¬Æ

url key :

rat-kim1-picokine-trade-elisa-kit-ek0882-boster

product category :

Picokine ELISA Kits

gene name :

HAVCR1

price :

380

assay range :

31.2 pg/ml - 2,000 pg/ml

description :

Rat KIM1 ELISA Kit PicoKine (96 Tests). Quantitate Rat Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml.

short description :

Rat KIM1 ELISA Kit PicoKine (96 Tests). Quantitate Rat Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

O54947

immunogen :

Expression system for standard: NS0; Immunogen sequence: S18-V238

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<2 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

applications :

ELISA

reactivity :

Rat

images :

/e/k/ek0882_1.png

image labels :

Rat KIM1 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.055 0.105 0.271 0.482 0.760 1.200 1.838

created at :

5/10/14 9:17

updated at :

3/2/22 2:54

meta title :

Rat KIM1 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Rat KIM1 ELISA Kit PicoKine, Bosterbio High Sensitivity

meta description :

Rat KIM1 ELISA Kit PicoKine (96 Tests). Quantitate Rat Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml.

rating value :

anticoagulant :

heparin or EDTA

assay procedure step 1 :

2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml

background :

KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rat is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the rat Tim1 gene and database analysis, was mapped the HAVCR1 gene to 5q33.2.

research category :

Adaptive Immunity,Host Virus Interaction,Immune System Diseases,Immunology,Interspecies Interaction,Microbiology,Non-Cd,T Cells

synonyms :

Hepatitis A virus cellular receptor 1 homolog;HAVcr-1;Kidney injury molecule 1;KIM-1;T cell immunoglobulin and mucin domain-containing protein 1;TIMD-1;Havcr1;Kim1;

gene full name :

Hepatitis A virus cellular receptor 1 homolog

molecular weight :

33964 MW

protein function :

May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair (By similarity).

subcellular localization :

Membrane ; Single-pass type I membrane protein .

tissue specificity :

Expressed at a low level in normal kidney but are increased dramatically in postischemic kidney. Expressed in proliferating bromodeoxyuridine-positive and dedifferentiated vimentin-positive epithelial cells in regenerating proximal tubules.

protein name :

Hepatitis A virus cellular receptor 1

sequence similarities :

Belongs to the immunoglobulin superfamily. TIM family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 87 272 938 86 293 1026 Standard deviation 3.82 20.67 59.09 3.95 23.14 84.13 CV (%) 4.4% 7.6% 7.9% 4.6% 7.9% 8.2%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 87 94 102 93 94 5.33 5.6% Sample 2 272 238 277 253 260 15.54 5.9% Sample 3 938 849 928 895 902 34.74 3.8% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

22898836

template :

elisa-kits

more info or order :

Boster product webpage