Human HSP27 / Heat Shock Protein 27 PicoKine ELISA Kit

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human HSP27 / Heat Shock Protein 27 PicoKine ELISA Kit

catalog :

EK0881

quantity :

96 wells/kit, with removable strips.

citations: 1

Reference
Jan Ankersmit H, Nickl S, Hoeltl E, Toepker M, Lambers C, Mitterbauer A, et al. Increased serum levels of HSP27 as a marker for incipient chronic obstructive pulmonary disease in young smokers. Respiration. 2012;83:391-9 pubmed publisher

product information

sku :

EK0881

status :

Enabled

name :

Human HSP27 / Heat Shock Protein 27 PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

HSPB1

assay range :

62.5pg/ml-4000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human Hsp27/HSPB1 with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma(heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Hsp27/HSPB1 tissue specificity: Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human HSP27. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P04792

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: T2-K205

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<5pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma(heparin, EDTA).

applications :

ELISA

reactivity :

Human

image labels :

Human HSP27 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml

background :

Hsp27 is a chaperone of the sHsp(small heat shock protein) group among ubiquitin, alpha-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kDa.

research category :

Contractility, Heat Shock Proteins, Protein Trafficking, Signal Transduction, Tumor Biomarkers

synonyms :

Heat shock protein beta-1;HspB1;28 kDa heat shock protein;Estrogen-regulated 24 kDa protein;Heat shock 27 kDa protein;HSP 27;Stress-responsive protein 27;SRP27;HSPB1;HSP27, HSP28;

gene full name :

Heat shock protein beta-1

molecular weight :

22783 MW

protein function :

Involved in stress resistance and actin organization.

subcellular localization :

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

tissue specificity :

Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

protein name :

Heat shock protein beta-1

sequence similarities :

Belongs to the small heat shock protein (HSP20) family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 82 492 1757 81 468 1729 Standard deviation 3.44 26.07 110.69 4.53 33.69 121.03 CV(%) 4.2% 5.3% 7.2%% 5.6% 7.2% 7%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 88 78 91 84 5.06 6% Sample 2 492 526 485 501 501 15.5 3% Sample 3 1757 1541 1607 1549 1613 86.67 5.3% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 4000 2000 4000 2000 4000 2000 4000 O.D. 0.099 0.119 0.173 0.253 0.384 0.697 1.127 1.796

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53