Mouse KIM1 / TIM1 ELISA Kit PicoKine¬Æ | Boster EK0880 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse KIM1 / TIM1 ELISA Kit PicoKine¬Æ

catalog :

EK0880

more info or order :

Boster product webpage

citations: 1

Reference
Sabbisetti V, Ito K, Wang C, Yang L, Mefferd S, Bonventre J. Novel assays for detection of urinary KIM-1 in mouse models of kidney injury. Toxicol Sci. 2013;131:13-25 pubmed publisher

image

image 1 :

Mouse KIM1 PicoKine ELISA Kit standard curve

product information

sku :

EK0880

status :

name :

Mouse KIM1 / TIM1 ELISA Kit PicoKine¬Æ

url key :

mouse-kim1-picokine-trade-elisa-kit-ek0880-boster

product category :

Picokine ELISA Kits

gene name :

HAVCR1

price :

380

assay range :

31.2 pg/ml - 2,000 pg/ml

description :

Mouse KIM1 / TIM1 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml.

short description :

Mouse KIM1 / TIM1 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

Q3V033

immunogen :

Expression system for standard: NS0; Immunogen sequence: Y22-T212

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<2 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

applications :

ELISA

reactivity :

Mouse

images :

/e/k/ek0880.png

image labels :

Mouse KIM1 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.061 0.104 0.172 0.294 0.520 0.977 1.756

created at :

5/10/14 9:17

updated at :

3/2/22 2:54

meta title :

Mouse KIM1 / TIM1 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Mouse KIM1 ELISA Kit PicoKine, Bosterbio High Sensitivity

meta description :

Mouse KIM1 / TIM1 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml.

rating value :

100

anticoagulant :

heparin or EDTA

assay procedure step 1 :

2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml

background :

KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.

research category :

Adaptive Immunity,Host Virus Interaction,Immune System Diseases,Immunology,Interspecies Interaction,Microbiology,Non-Cd,T Cells

synonyms :

Hepatitis A virus cellular receptor 1 homolog ;Hepatitis A virus cellular receptor 1, isoform CRA_b ;Havcr1 ;mCG_117142 ;

gene full name :

hepatitis A virus cellular receptor 1

molecular weight :

30968 MW

protein name :

Hepatitis A virus cellular receptor 1 homolog

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 87 279 842 85 261 792 Standard deviation 6.87 14.78 36.2 7.31 14.87 38.8 CV (%) 7.9% 5.3% 4.3% 8.6% 5.7% 4.9%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 87 90 90 96 90 3.26 3.6% Sample 2 279 266 249 284 269 13.53 5% Sample 3 842 734 811 767 788 41.23 5.2% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

polyclonal antibody from goat polyclonal antibody from goat

normal range citations :

23019274

template :

elisa-kits

more info or order :

Boster product webpage