ELISA/assay

Human PECAM-1/CD31 ELISA Kit PicoKine®

EK0873

1

Human PECAM-1/CD31 ELISA Kit PicoKine®

human-pecam-1-cd31-picokine-trade-elisa-kit-ek0873-boster

Picokine ELISA Kits

PECAM1

420

156 pg/ml - 10,000 pg/ml

Human PECAM-1/CD31 ELISA Kit PicoKine (96 Tests). Quantitate Human PECAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Human PECAM-1/CD31 ELISA Kit PicoKine (96 Tests). Quantitate Human PECAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

P16284

Expression system for standard: CHO; Immunogen sequence: Q28-K601

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

ELISA

Human

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Human PECAM-1/CD31 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.144 0.219 0.375 0.645 1.049 1.614 2.074

5/10/14 9:17

3/2/22 2:54

Human PECAM-1/CD31 ELISA Kit Price PicoKine® BosterBio

Human PECAM-1/CD31 ELISA Kit, PECAM 1 ELISA, Bosterbio High Sensitivity

Human PECAM-1/CD31 ELISA Kit PicoKine (96 Tests). Quantitate Human PECAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

100

heparin or EDTA

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

Human platelet/endothelial cell adhesion molecule-1 (PECAM1), an important member of the immunoglobulin gene superfamily, is widely distributed on cells of the vascular system and mediates cellular interactions through both homophilic and heterophilic adhesive mechanisms. The function of PECAM1 in vitro has begun to be understood, but its function in vivo is yet to be established.1 The PECAM1 locus is on the long arm of chromosome 17, in the region q23-qter. To confirm this observation and obtain a more precise localization of the PECAM1 locus, fluorescence in situ hybridization was conducted. Together our data allowed assignment of the PECAM1 locus to the region 17q23.2 To examine the functional role of PECAM-1 in regulating platelet-collagen interactions, 2 different approaches were applied using recombinant human PECAM-1-immunoglobulin chimeras and platelets derived from PECAM-1-deficient mice.3 PECAM-1, an integral membrane protein with an essential role in TEM, is found in this compartment and constitutively recycles evenly along endothelial cell borders.4 The standard used in this kit is the product of gene recombinant expression (extracellular part), consisting of 574 amino acids (Q28-K601) with the molecular weight of 64.5KDa. After glycosylating, its molecular weight changes to 90-100KDa.

Angiogenesis,Cardiovascular,Cell Adhesion,Cell Adhesion Molecules,Cell Type Markers,Cytoskeleton / Ecm,Immunology,Signal Transduction

Platelet endothelial cell adhesion molecule;PECAM-1;EndoCAM;GPIIA';PECA1;CD31;PECAM1;

Platelet endothelial cell adhesion molecule

82536 MW

Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

Isoform Long: Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.

Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level).

Platelet endothelial cell adhesion molecule

Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 292 1327 4781 278 1442 4709 Standard deviation 11.68 87.58 310.76 13.62 114.94 367.3 CV (%) 4% 6.6% 6.5% 4.9% 8.2% 7.8%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 292 286 327 276 295 19.2 6.5% Sample 2 1327 1428 1563 1332 1412 95.76 6.7% Sample 3 4781 4159 4685 4186 4452 282.45 6.3% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

11571836

elisa-kits