Human DKK-4 / Dickkopf 4 ELISA Kit PicoKine® | Boster EK0868 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human DKK-4 / Dickkopf 4 ELISA Kit PicoKine®

catalog :

EK0868

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK0868

Product Name :

Human DKK-4 / Dickkopf 4 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human DKK-4 / Dickkopf 4 ELISA Kit PicoKine® (96 Tests). Quantitate Human DKK4 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

DKK4

Uniprot ID :

Q9UBT3

Immunogen :

Expression system for standard: NS0; Immunogen sequence: L19-L224

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

dickkopf WNT signaling pathway inhibitor 4

Synonyms :

Dickkopf-related protein 4; Dickkopf-4; Dkk-4; hDkk-4; Dickkopf-related protein 4 short form; DKK4

Protein Function :

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).

Subcellular Localization :

Secreted.

Tissue Specificity :

Expressed in cerebellum, T-cells, esophagus and lung.

Background :

DKK4 is a membersof the Dickkopf family which are negative regulators of the growth-promoting Wnt / beta-catenin signaling pathway. By FISH, the DKK4 gene was mapped to chromosome 8p11.2-p11.1. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. The expression of DKK4 was significantly reduced in hepatocellular carcinomas (HCCs), particularly in HCCs that showed cytoplasmic beta- catenin accumulation. DKK4 expression was also reduced in HCC cell lines. Ectopic expression of DKK4 in 2 HCC cell lines reduced beta-catenin and cyclin D1 protein levels and attenuated beta-catenin activity in reporter gene assays.

Research Category :

Secreted, Signaling Pathways, Stem Cells