Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine¬Æ | Boster EK0867 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine¬Æ

catalog :

EK0867

more info or order :

Boster product webpage

citations: 3

Reference
Yeremenko N, Zwerina K, Rigter G, Pots D, Fonseca J, Zwerina J, et al. Tumor necrosis factor and interleukin-6 differentially regulate Dkk-1 in the inflamed arthritic joint. Arthritis Rheumatol. 2015;67:2071-5 pubmed publisher
Tian J, Xu X, Shen L, Yang Y, Zhu R, Shuai B, et al. Association of serum Dkk-1 levels with ?-catenin in patients with postmenopausal osteoporosis. J Huazhong Univ Sci Technolog Med Sci. 2015;35:212-218 pubmed publisher
Dong L, Qu L, Chu L, Zhang X, Liu Y. Serum level of DKK-1 and its prognostic potential in non-small cell lung cancer. Diagn Pathol. 2014;9:52 pubmed publisher

image

image 1 :

Human DKK-1 PicoKine ELISA Kit standard curve

product information

sku :

EK0867

status :

name :

Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine¬Æ

url key :

human-dkk-1-picokine-trade-elisa-kit-ek0867-boster

product category :

Picokine ELISA Kits

gene name :

DKK1

price :

369

assay range :

62.5 pg/ml - 4,000 pg/ml

description :

Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine (96 Tests). Quantitate Human DKK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

short description :

Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine (96 Tests). Quantitate Human DKK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

I1W660

immunogen :

Expression system for standard: sf21; Immunogen sequence: T32-H266

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:20, concentration in serum is around 9ng/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0867.png

image labels :

Human DKK-1 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.012 0.107 0.187 0.402 0.747 1.368 1.899 2.406

created at :

7/8/14 16:17

updated at :

3/2/22 2:54

meta title :

Human DKK-1 / Dickkopf 1 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human DKK-1 ELISA Kit PicoKine, Bosterbio High Sensitivity

meta description :

Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine (96 Tests). Quantitate Human DKK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

rating value :

100

anticoagulant :

heparin or EDTA

assay procedure step 1 :

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

background :

Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

research category :

Cardiovascular,Developmental Biology,Embryogenesis,Immunology,Secreted,Signaling Pathways,Stem Cells

synonyms :

Dickkopf-like protein 1 ;DKK1 ;

gene full name :

dickkopf WNT signaling pathway inhibitor 1

molecular weight :

28672 MW

protein name :

Dickkopf-related protein 1

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 660 1140 103 594 1134 Standard deviation 5.72 47.52 66.12 6.59 43.36 82.78 CV (%) 5.3% 7.2% 5.8% 6.4% 7.3% 7.3%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 125 119 115 116 6.17 5.3% Sample 2 660 564 616 629 617 34.65 5.6% Sample 3 1140 1087 1155 1110 1123 26.35 2.3% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

25941031, 25877354, 24612589

template :

elisa-kits

more info or order :

Boster product webpage