Human IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine¬Æ

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine¬Æ

catalog :

EK0864

more info or order :

Boster product webpage

citations: 1

Reference
Rasool R, Ashiq I, Shera I, Yousuf Q, Shah Z. Study of serum interleukin (IL) 18 and IL-6 levels in relation with the clinical disease severity in chronic idiopathic urticaria patients of Kashmir (North India). Asia Pac Allergy. 2014;4:206-11 pubmed publisher

image

image 1 :

Human IL-18 PicoKine ELISA Kit standard curve

product information

sku :

EK0864

status :

name :

Human IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine¬Æ

url key :

human-il-18-picokine-trade-elisa-kit-ek0864-boster

product category :

Picokine ELISA Kits

gene name :

IL18

price :

400

assay range :

31.2 pg/ml - 2,000 pg/ml

description :

Human IL-18 ELISA Kit PicoKine (96 Tests). Quantitate Human IL18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

short description :

Human IL-18 ELISA Kit PicoKine (96 Tests). Quantitate Human IL18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

Q14116

immunogen :

Expression system for standard: E.coli; Immunogen sequence: Y33-D197

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0864_1.png

image labels :

Human IL-18 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.033 0.105 0.132 0.216 0.364 0.676 1.235 1.955

created at :

5/10/14 16:17

updated at :

3/2/22 2:54

meta title :

Human IL-18/IL-1F4/Interleukin-18 ELISA Kit Price PicoKine¬Æ

meta keyword :

Human IL-18 ELISA Kit PicoKine, Bosterbio High Sensitivity

rating value :

98.7

anticoagulant :

heparin or EDTA

assay procedure step 1 :

2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml

background :

Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

research category :

Angiogenesis,Cancer,Cancer Metabolism,Cytokines,Immunology,Innate Immunity,Interleukins,Invasion/Microenvironment,Metabolism,Metabolism Processes,Pathways And Processes,Response To Hypoxia,Tumor Immunology

synonyms :

Interleukin-18;IL-18;Iboctadekin;Interferon gamma-inducing factor;IFN-gamma-inducing factor;Interleukin-1 gamma;IL-1 gamma;IL18;IGIF, IL1F4;

gene full name :

Interleukin-18

molecular weight :

22326 MW

protein function :

Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

subcellular localization :

Secreted.

protein name :

Interleukin-18

sequence similarities :

Belongs to the IL-1 family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 247 520 36 254 548 Standard deviation 2.4 13.83 21.84 2.66 16.25 31.78 CV (%) 7.3% 5.6% 4.2% 7.4% 6.4% 5.8%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 32 33 31 32 0.82 2.5% Sample 2 247 212 221 232 228 13.05 5.7% Sample 3 520 533 562 546 478 31.15 5.4% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse monoclonal antibody from mouse

normal range citations :

25379480

template :

elisa-kits

more info or order :

Boster product webpage