Human PAI-1 ELISA Kit PicoKine¬Æ | Boster EK0859 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human PAI-1 ELISA Kit PicoKine¬Æ

catalog :

EK0859

more info or order :

Boster product webpage

citations: 1

Reference
Zhu H, Wang Z, Xue X, Zhang P, Yang C, Su B. [Lower grade chronic inflammation is associated with obstructive sleep apnea syndrome in type 2 diabetes mellitus]. Zhonghua Jie He He Hu Xi Za Zhi. 2014;37:411-5 pubmed

image

image 1 :

Human PAI-1 PicoKine ELISA Kit standard curve

product information

sku :

EK0859

status :

name :

Human PAI-1 ELISA Kit PicoKine¬Æ

url key :

human-pai-1-picokine-trade-elisa-kit-ek0859-boster

product category :

Picokine ELISA Kits

gene name :

SERPINE1

price :

400

assay range :

156 pg/ml - 10,000 pg/ml

description :

Human PAI-1 ELISA Kit PicoKine (96 Tests). Quantitate Human SERPINE1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml.

short description :

Human PAI-1 ELISA Kit PicoKine (96 Tests). Quantitate Human SERPINE1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P05121

immunogen :

Expression system for standard: NS0; Immunogen sequence: S22-P402

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate ).

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0859.png

image labels :

Human PAI-1 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.155 0.279 0.420 0.649 1.043 1.460 2.024

created at :

5/10/14 23:17

updated at :

3/2/22 2:54

meta title :

Human PAI-1 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human PAI-1 ELISA Kit, PAI 1/PAI ELISA, Bosterbio High Sensitivity

meta description :

Human PAI-1 ELISA Kit PicoKine (96 Tests). Quantitate Human SERPINE1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml.

rating value :

98.6

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

background :

Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots). It is a serine protease inhibitor (serpin) protein (SERPINE1). PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome (7q21.3-q22).1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis (a tendency to hemorrhage). PAI-1 is present in increased levels in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43KDa.

research category :

Angiogenesis,Cardiovascular

synonyms :

Plasminogen activator inhibitor 1;PAI;PAI-1;Endothelial plasminogen activator inhibitor;Serpin E1;SERPINE1;PAI1, PLANH1;

gene full name :

Plasminogen activator inhibitor 1

molecular weight :

45060 MW

protein function :

Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.

subcellular localization :

Secreted.

tissue specificity :

Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

protein name :

Plasminogen activator inhibitor 1

sequence similarities :

Belongs to the serpin family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 1208 5496 198 1244 5980 Standard deviation 12.38 71.27 423.19 15.04 95.78 520.26 CV (%) 6.1% 7.7%% 7.7% 7.6% 7.7% 8.7%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 218 218 234 218 10.96 5% Sample 2 1208 1308 1324 1403 1310 69.37 5.2% Sample 3 5496 5184 6077 5696 5613 324 5.7% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

25200039

template :

elisa-kits

more info or order :

Boster product webpage