ELISA/assay

Human TNFSF4/OX40L ELISA Kit PicoKine®

EK0857

1

Human TNFSF4/OX40L ELISA Kit PicoKine®

human-tnfsf4-ox40l-picokine-trade-elisa-kit-ek0857-boster

Picokine ELISA Kits

TNFSF4

369

156 pg/ml - 10,000 pg/ml

Human TNFSF4/OX40L ELISA Kit PicoKine (96 Tests). Quantitate Human TNFSF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Human TNFSF4/OX40L ELISA Kit PicoKine (96 Tests). Quantitate Human TNFSF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

P23510

Expression system for standard: NS0; Immunogen sequence: Q51-L183

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit.

ELISA

Human

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Human TNFSF4/OX40L PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.138 0.238 0.365 0.657 1.151 1.548 2.030

5/10/14 23:17

3/2/22 2:54

Human TNFSF4/OX40L ELISA Kit Price PicoKine® BosterBio

Human TNFSF4/OX40L ELISA Kit, Bosterbio High Sensitivity

Human TNFSF4/OX40L ELISA Kit PicoKine (96 Tests). Quantitate Human TNFSF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

95

heparin or EDTA

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 313 pg/ml, 156 pg/ml

OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, the mouse Tnfsf4 gene is mapped to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene. OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.

Adaptive Immunity,Autoimmune,B Cells,Cell Type Markers,Cytokines,Immune System Diseases,Immunology,Innate Immunity,T Cells,Tnf Superfamily

Tumor necrosis factor ligand superfamily member 4;Glycoprotein Gp34;OX40 ligand;OX40L;TAX transcriptionally-activated glycoprotein 1;CD252;TNFSF4;TXGP1;

Tumor necrosis factor ligand superfamily member 4

21050 MW

Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.

Membrane; Single-pass type II membrane protein.

Tumor necrosis factor ligand superfamily member 4

Belongs to the tumor necrosis factor family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 1225 4641 228 1277 4885 Standard deviation 8.56 51.45 305.70 12.54 72.78 365.77 CV (%) 4.1% 4.2% 6.6% 5.5% 5.7% 7.5%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 197 197 202 201 4.91 2.4% Sample 2 1225 1132 1200 1122 1169 43.79 3.7% Sample 3 4641 5209 4997 5343 5047 265.13 5.2% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

18843780

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