Rat Lipocalin-2/NGAL ELISA Kit PicoKine¬Æ | Boster EK0855 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Rat Lipocalin-2/NGAL ELISA Kit PicoKine¬Æ

catalog :

EK0855

more info or order :

Boster product webpage

citations: 1

Reference
Martines M, Mendes M, Shimizu M, Melo Rodrigues V, de Castro I, Filho S, et al. Effects of Schizolobium parahyba extract on experimental Bothrops venom-induced acute kidney injury. PLoS ONE. 2014;9:e86828 pubmed publisher

image

image 1 :

Rat Lipocalin-2/NGAL PicoKine ELISA Kit standard curve

product information

sku :

EK0855

status :

name :

Rat Lipocalin-2/NGAL ELISA Kit PicoKine¬Æ

url key :

rat-lipocalin-2-ngal-picokine-trade-elisa-kit-ek0855-boster

product category :

Picokine ELISA Kits

gene name :

LCN2

price :

400

assay range :

78 pg/ml - 5,000 pg/ml

description :

Rat Lipocalin-2/NGAL ELISA Kit PicoKine (96 Tests). Quantitate Rat Lcn2 in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 10pg/ml.

short description :

Rat Lipocalin-2/NGAL ELISA Kit PicoKine (96 Tests). Quantitate Rat Lcn2 in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P30152

immunogen :

Expression system for standard: NS0; Immunogen sequence: Q21-N198

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, serum, plasma (heparin) and urine.

applications :

ELISA

reactivity :

Rat

images :

/e/k/ek0855.png

image labels :

Rat Lipocalin-2/NGAL PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.067 0.135 0.181 0.312 0.484 0.780 1.249 1.737

created at :

5/10/14 23:17

updated at :

3/2/22 2:54

meta title :

Rat Lipocalin-2/NGAL ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Rat Lipocalin-2/NGAL ELISA Kit, Lipocalin 2 ELISA

meta description :

Rat Lipocalin-2/NGAL ELISA Kit PicoKine (96 Tests). Quantitate Rat Lcn2 in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 10pg/ml.

rating value :

97.5

anticoagulant :

heparin

assay procedure step 1 :

5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 313 pg/ml, 156 pg/ml, 78 pg/ml

background :

Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant rat NGAL, consisting of 177 amino acids with the molecular mass of 21kDa.

research category :

Immunoglobulins,Immunology

synonyms :

Neutrophil gelatinase-associated lipocalin;NGAL;Alpha-2-microglobulin-related protein;Alpha-2U globulin-related protein;Lipocalin-2;Siderocalin LCN2;p25;Lcn2;

gene full name :

Neutrophil gelatinase-associated lipocalin

molecular weight :

22476 MW

protein function :

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth (By similarity).

subcellular localization :

Secreted . Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.

protein name :

Neutrophil gelatinase-associated lipocalin

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 121 750 3188 126 727 3167 Standard deviation 6.05 50.25 168.96 7.3 56.7 177.35 CV (%) 5% 6.7% 5.3% 5.8% 7.8% 5.6%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 121 118 102 117 114 7.36 6.4% Sample 2 750 751 693 740 733 23.77 3.2% Sample 3 3188 2684 2847 3144 2965 208.95 7% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

24551041

template :

elisa-kits

more info or order :

Boster product webpage