Mouse CD10/Neprilysin ELISA Kit PicoKine¬Æ | Boster EK0852 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse CD10/Neprilysin ELISA Kit PicoKine¬Æ

catalog :

EK0852

more info or order :

Boster product webpage

citations: 1

Reference
Sasaki T, Kuniyasu H, Luo Y, Fujiwara R, Kitayoshi M, Tanabe E, et al. Serum CD10 is associated with liver metastasis in colorectal cancer. J Surg Res. 2014;192:390-4 pubmed publisher

image

image 1 :

Mouse Neprilysin/CD10 PicoKine ELISA Kit standard curve

product information

sku :

EK0852

status :

name :

Mouse CD10/Neprilysin ELISA Kit PicoKine¬Æ

url key :

mouse-cd10-neprilysin-picokine-trade-elisa-kit-ek0852-boster

product category :

Picokine ELISA Kits

gene name :

MME

price :

369

assay range :

93.7 pg/ml - 6,000 pg/ml

description :

Mouse CD10/Neprilysin ELISA Kit PicoKine (96 Tests). Quantitate Mouse Mme in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

short description :

Mouse CD10/Neprilysin ELISA Kit PicoKine (96 Tests). Quantitate Mouse Mme in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

Q61391

immunogen :

Expression system for standard: sf21; Immunogen sequence: Y52-W750

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:1, concentration in serum is around 200pg/ml

applications :

ELISA

reactivity :

Mouse

images :

/e/k/ek0852.png

image labels :

Mouse Neprilysin/CD10 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 93.7 187.5 375 750 13000 3000 6000 O.D. 0.105 0.209 0.274 0.421 0.784 1.358 1.967 2.481

created at :

5/10/14 23:17

updated at :

3/2/22 2:54

meta title :

Mouse CD10/Neprilysin ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Mouse Neprilysin/CD10 ELISA Kit, Bosterbio High Sensitivity

meta description :

Mouse CD10/Neprilysin ELISA Kit PicoKine (96 Tests). Quantitate Mouse Mme in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

rating value :

100

anticoagulant :

heparin or EDTA

assay procedure step 1 :

6,000 pg/ml, 3,000 pg/ml, 1,500 pg/ml, 750 pg/ml, 375 pg/ml, 187.5 pg/ml, 93.7pg/m

background :

Neprilysin, also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), CD10, and common acute lymphoblastic leukemia antigen (CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease, this gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, Neprilysin is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. Neprilysin has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

research category :

Angiogenesis,Atherosclerosis,Cancer,Cardiovascular,Cytoskeleton / Ecm,Ecm Enzymes,Extracellular Matrix,Invasion/Microenvironment,Signal Transduction,Tumor Biomarkers

synonyms :

Neprilysin;3.4.24.11 ;Atriopeptidase;Enkephalinase;Neutral endopeptidase 24.11;NEP;Neutral endopeptidase;Skin fibroblast elastase;SFE;CD10;Mme;

gene full name :

Neprilysin

molecular weight :

85702 MW

protein function :

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) (By similarity). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).

subcellular localization :

Cell membrane; Single-pass type II membrane protein.

protein name :

Neprilysin

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 165 1021 3228 175 951 3447 Standard deviation 7.26 41.86 216.27 4.7%2 57.4%5 255.07 CV (%) 4.4% 4.1% 6.7% 4.7% 5.8% 7.4%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 165 178 186 177 176 7.5 4.2% Sample 2 1021 907 995 1027 987 48 4.8% Sample 3 3228 3394 3345 3463 3357 85.71 2.5% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from rat polyclonal antibody from goat

normal range citations :

24972738

template :

elisa-kits

more info or order :

Boster product webpage