Human CD10/Neprilysin ELISA Kit PicoKine¬Æ | Boster EK0851 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human CD10/Neprilysin ELISA Kit PicoKine¬Æ

catalog :

EK0851

more info or order :

Boster product webpage

citations: 1

Reference
Sasaki T, Kuniyasu H, Luo Y, Fujiwara R, Kitayoshi M, Tanabe E, et al. Serum CD10 is associated with liver metastasis in colorectal cancer. J Surg Res. 2014;192:390-4 pubmed publisher

image

image 1 :

Human CD10/Neprilysin PicoKine ELISA Kit standard curve

product information

sku :

EK0851

status :

name :

Human CD10/Neprilysin ELISA Kit PicoKine¬Æ

url key :

human-cd10-neprilysin-picokine-trade-elisa-kit-ek0851-boster

product category :

Picokine ELISA Kits

gene name :

MME

price :

369

assay range :

125 pg/ml - 8,000 pg/ml

description :

Human CD10/Neprilysin ELISA Kit PicoKine (96 Tests). Quantitate Human MME in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

short description :

Human CD10/Neprilysin ELISA Kit PicoKine (96 Tests). Quantitate Human MME in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P08473

immunogen :

Expression system for standard: CHO; Immunogen sequence: Y52-W750

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

images :

/antibody/ek0851-2-ELISA-human-cd10-neprilysin-picokine-elisa-kit.jpg

image labels :

Human CD10/Neprilysin PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.097 0.148 0.196 0.319 0.462 0.858 1.328 1.965

created at :

5/10/14 23:17

updated at :

3/2/22 2:54

meta title :

Human CD10/Neprilysin ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human CD10/Neprilysin ELISA Kit, Bosterbio High Sensitivity

meta description :

Human CD10/Neprilysin ELISA Kit PicoKine (96 Tests). Quantitate Human MME in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

rating value :

97.5

anticoagulant :

heparin or EDTA

assay procedure step 1 :

8,000 pg/ml, 4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml

background :

CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), Neprilysin, or common acute lymphoblastic leukemia antigen (CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

research category :

Angiogenesis,Atherosclerosis,Cancer,Cardiovascular,Cytoskeleton / Ecm,Ecm Enzymes,Extracellular Matrix,Invasion/Microenvironment,Signal Transduction,Tumor Biomarkers

synonyms :

Neprilysin;3.4.24.11 ;Atriopeptidase;Common acute lymphocytic leukemia antigen;CALLA;Enkephalinase;Neutral endopeptidase 24.11;NEP;Neutral endopeptidase;Skin fibroblast elastase;SFE;CD10;MME;EPN;

gene full name :

Neprilysin

molecular weight :

85514 MW

protein function :

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).

subcellular localization :

Cell membrane; Single-pass type II membrane protein.

protein name :

Neprilysin

sequence similarities :

Belongs to the peptidase M13 family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 201 2166 4316 216 881 4302 Standard deviation 14.87 51.62 233.06 17.92 60.78 305.44 CV (%) 7.4% 5.4% 5.4% 8.3% 6.9% 7.1%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 201 170 181 181 183 11.18 6.1% Sample 2 956 1058 1011 1064 1022 43.4 4.2% Sample 3 4316 4490 4532 4435 4443 81.12 1.8% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

polyclonal antibody from goat polyclonal antibody from goat

normal range citations :

24972738

template :

elisa-kits

more info or order :

Boster product webpage