product summary
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company name :
Boster
product type :
ELISA/assay
product name :
Human MPO/Myeloperoxidase ELISA Kit PicoKine®
catalog :
EK0850
more info or order :
citations: 1
Reference
Czepiel J, Biesiada G, Brzozowski T, Ptak Belowska A, Perucki W, Birczyńska M, et al. The role of local and systemic cytokines in patients infected with Clostridium difficile. J Physiol Pharmacol. 2014;65:695-703 pubmed
image
image 1 :
Boster EK0850 image 1
Human MPO PicoKine ELISA Kit standard curve
product information
sku :
EK0850
status :
1
name :
Human MPO/Myeloperoxidase ELISA Kit PicoKine®
url key :
human-mpo-picokine-trade-elisa-kit-ek0850-boster
product category :
Picokine ELISA Kits
gene name :
MPO
price :
400
assay range :
312 pg/ml - 20,000 pg/ml
description :
Human MPO/Myeloperoxidase ELISA Kit PicoKine (96 Tests). Quantitate Human MPO in cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml.
short description :
Human MPO/Myeloperoxidase ELISA Kit PicoKine (96 Tests). Quantitate Human MPO in cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml.
size :
96 wells/kit, with removable strips.
uniprot id :
P05164
immunogen :
Expression system for standard: NS0; Immunogen sequence: A49-S745
storage :
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
cross reactivity :
There is no detectable cross-reactivity with other relevant proteins.
sensitivity :
<10 pg/ml
kit components :
20 ng/tube
sample type :
cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine.
application details :
Dilution ratio of 1:200, concentration in serum is around 600ng/ml
applications :
ELISA
reactivity :
Human
images :
/e/k/ek0850.png
image labels :
Human MPO PicoKine ELISA Kit standard curve
sample data :
Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.089 0.280 0.398 0.602 0.884 1.128 1.575 1.926
created at :
5/10/14 23:17
updated at :
3/2/22 2:54
meta title :
Human MPO/Myeloperoxidase ELISA Kit Price PicoKine® BosterBio
meta keyword :
Human MPO ELISA Kit, 84 kDa myeloperoxidase ELISA Kit
meta description :
Human MPO/Myeloperoxidase ELISA Kit PicoKine (96 Tests). Quantitate Human MPO in cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml.
rating value :
99.5
anticoagulant :
heparin or EDTA
assay procedure step 1 :
20,000 pg/ml, 10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml
background :
Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697 (A49-S745) amino acids with the molecular mass of 80KDa.
competitor equivalent skus :
sc 52076 sc 59600 sc 51741 sc 52707 sc 66106 sc 66107
research category :
Cancer,Cancer Metabolism,Cardiovascular,Immunology,Innate Immunity,Macrophage / Inflamm.,Metabolism,Pathways And Processes,Redox Metabolism,Vasculature
synonyms :
Myeloperoxidase;MPO;1.11.2.2;Myeloperoxidase;89 kDa myeloperoxidase;84 kDa myeloperoxidase;Myeloperoxidase light chain;Myeloperoxidase heavy chain;MPO;
gene full name :
Myeloperoxidase
molecular weight :
83869 MW
protein function :
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
subcellular localization :
Lysosome
protein name :
Myeloperoxidase
sequence similarities :
Belongs to the peroxidase family. XPO subfamily.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 580 2272 9558 587 2421 9230 Standard deviation 42.92 154.49 401.43 48.13 191.25 507.65 CV (%) 7.4% 6.8% 4.2% 8.2% 7.9% 5.5%
reproducibility :
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 580 586 560 663 597 39.16 6.5% Sample 2 2272 2188 2044 2304 2202 100.57 4.5% Sample 3 9558 8956 8970 10074 9389 463.9 4.9% *number of samples for each test n=16.
tmb incubation time :
15-25min
principle :
monoclonal antibody from mouse polyclonal antibody from goat
normal range citations :
25371529
template :
elisa-kits
more info or order :
company information
Boster
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
https://www.bosterbio.com
925.485.4527
headquarters: USA
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