Human TNFSF11/RANKL ELISA Kit PicoKine¬Æ | Boster EK0842 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human TNFSF11/RANKL ELISA Kit PicoKine¬Æ

catalog :

EK0842

more info or order :

Boster product webpage

citations: 1

Reference
Xu S, Wang Y, Lu J, Xu J. Osteoprotegerin and RANKL in the pathogenesis of rheumatoid arthritis-induced osteoporosis. Rheumatol Int. 2012;32:3397-403 pubmed publisher

image

image 1 :

Human TNFSF11/RANKL PicoKine ELISA Kit standard curve

product information

sku :

EK0842

status :

name :

Human TNFSF11/RANKL ELISA Kit PicoKine¬Æ

url key :

human-tnfsf11-rankl-picokine-trade-elisa-kit-ek0842-boster

product category :

Picokine ELISA Kits

gene name :

TNFSF11

price :

369

assay range :

78 pg/ml - 5,000 pg/ml

description :

Human TNFSF11/RANKL ELISA Kit PicoKine (96 Tests). Quantitate Human TNFSF11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

short description :

Human TNFSF11/RANKL ELISA Kit PicoKine (96 Tests). Quantitate Human TNFSF11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

O14788

immunogen :

Expression system for standard: NS0; Immunogen sequence: G64-D245

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit.

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0842_1.png

image labels :

Human TNFSF11/RANKL PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.034 0.139 0.151 0.262 0.453 0.783 1.331 1.926

created at :

5/10/14 23:17

updated at :

3/2/22 2:54

meta title :

Human TNFSF11/RANKL ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human TNFSF11/RANKL ELISA Kit, Bosterbio High Sensitivity

meta description :

Human TNFSF11/RANKL ELISA Kit PicoKine (96 Tests). Quantitate Human TNFSF11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

rating value :

100

anticoagulant :

heparin or EDTA

assay procedure step 1 :

5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 313 pg/ml, 156 pg/ml, 78 pg/ml

background :

Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

research category :

Adaptive Immunity,Atherosclerosis,Cardiovascular,Cytokines,Cytoskeleton / Ecm,Extracellular Matrix,Immunology,Innate Immunity,Signal Transduction,Structures,Tnf Superfamily

synonyms :

Tumor necrosis factor ligand superfamily member 11;Osteoclast differentiation factor;ODF;Osteoprotegerin ligand;OPGL;Receptor activator of nuclear factor kappa-B ligand;RANKL;TNF-related activation-induced cytokine;TRANCE;CD254;Tumor necrosis factor ligand superfamily member 11, membrane form;Tumor necrosis factor ligand superfamily member 11, soluble form;TNFSF11;OPGL, RANKL, TRANCE;

gene full name :

Tumor necrosis factor ligand superfamily member 11

molecular weight :

35478 MW

protein function :

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

subcellular localization :

Isoform 1: Cell membrane; Single-pass type II membrane protein.

tissue specificity :

Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.

protein name :

Tumor necrosis factor ligand superfamily member 11

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 115 865 2242 114 797 2279 Standard deviation 5.52 53.63 105.37 4.72 58.97 116.22 CV (%) 4.8% 6.2% 4.7% 5.2% 7.4% 5.1%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 115 126 117 122 120 4.3 3.5% Sample 2 865 746 848 804 815 46.01 5.6% Sample 3 2242 2225 2256 2223 2236 13.46 0.6% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

polyclonal antibody from goat polyclonal antibody from goat

normal range citations :

22057136

template :

elisa-kits

more info or order :

Boster product webpage