Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine¬Æ

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine¬Æ

catalog :

EK0840

more info or order :

Boster product webpage

citations: 1

Reference
Zemanova P, Opatrny K, Opatrna S, Vit L, Sefrna F, Racek J. Tissue factor, its pathway inhibitor, and metabolic disturbances in long-term peritoneal dialysis. Kidney Blood Press Res. 2003;26:368-75 pubmed

image

image 1 :

Human TFPI PicoKine ELISA Kit standard curve

product information

sku :

EK0840

status :

name :

Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine¬Æ

url key :

human-tfpi-picokine-trade-elisa-kit-ek0840-boster

product category :

Picokine ELISA Kits

gene name :

TFPI

price :

369

assay range :

31.2 pg/ml - 2,000 pg/ml

description :

Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine (96 Tests). Quantitate Human TFPI in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.

short description :

Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine (96 Tests). Quantitate Human TFPI in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P10646

immunogen :

Expression system for standard: NS0; Immunogen sequence: D29-K282

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0840_1.png

image labels :

Human TFPI PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.043 0.069 0.119 0.167 0.283 0.515 0.944 1.764

created at :

5/10/14 23:17

updated at :

3/2/22 2:54

meta title :

Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit Price PicoKine¬Æ

meta keyword :

Human TFPI ELISA Kit, LACI ELISA Kit, Bosterbio High Sensitivity

meta description :

Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine (96 Tests). Quantitate Human TFPI in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.

rating value :

98.8

anticoagulant :

heparin or EDTA

assay procedure step 1 :

2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml

background :

TFPI (Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus.TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.

competitor equivalent skus :

sc 28862 sc 28861 sc 365920 sc 18712 sc 18714 sc 133139 sc 137126 sc 18713 sc 18716

research category :

Cardiovascular,Platelets

synonyms :

Tissue factor pathway inhibitor;TFPI;Extrinsic pathway inhibitor;EPI;Lipoprotein-associated coagulation inhibitor;LACI;TFPI;LACI, TFPI1;

gene full name :

Tissue factor pathway inhibitor

molecular weight :

35015 MW

protein function :

Inhibits factor X (X (a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.

subcellular localization :

Isoform Alpha: Secreted.

tissue specificity :

Mostly in endothelial cells.

protein name :

Tissue factor pathway inhibitor

sequence similarities :

Contains 3 BPTI/Kunitz inhibitor domains.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 238 848 55 237 897 Standard deviation 3.3 11.9 66.14 3.46 14.93 75.34 CV (%) 5.8% 5% 7.8% 6.3%% 6.3% 8.4%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 57 66 64 61 4.06 6.6% Sample 2 238 272 240 245 248 13.66 5.5% Sample 3 848 982 998 845 918 71.98 7.8% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

polyclonal antibody from goat polyclonal antibody from goat

normal range citations :

14610342

template :

elisa-kits

more info or order :

Boster product webpage