ELISA/assay

Human Kallistatin/Serpina4 ELISA Kit PicoKine®

EK0836

1

Human Kallistatin/Serpina4 ELISA Kit PicoKine®

human-kallistatin-serpina4-picokine-trade-elisa-kit-ek0836-boster

Picokine ELISA Kits

SERPINA4

399

156 pg/ml - 10,000 pg/ml

Human Kallistatin/Serpina4 ELISA Kit PicoKine (96 Tests). Quantitate Human SERPINA4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Human Kallistatin/Serpina4 ELISA Kit PicoKine (96 Tests). Quantitate Human SERPINA4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

P29622

Expression system for standard: NS0; Immunogen sequence: Q21-P427

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

ELISA

Human

/e/k/ek0836.png

Human Kallistatin/Serpina4 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.143 0.259 0.514 0.832 1.334 1.850 2.719

7/8/14 23:17

3/2/22 2:54

Human Kallistatin/Serpina4 ELISA Kit Price PicoKine®

Human Kallistatin/Serpina4 ELISA Kit, Bosterbio High Sensitivity

Human Kallistatin/Serpina4 ELISA Kit PicoKine (96 Tests). Quantitate Human SERPINA4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

100

heparin or EDTA

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.

Protease Inhibitors,Proteolysis / Ubiquitin,Serum Proteins

Kallistatin;Kallikrein inhibitor;Peptidase inhibitor 4;PI-4;Serpin A4;SERPINA4;KST, PI4;

Kallistatin

48542 MW

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.

Secreted.

Expressed by the liver and secreted in plasma.

Kallistatin

Belongs to the serpin family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1648 4379 232 1601 4027 Standard deviation 11.37 95.58 214.57 12.76 94.45 249.67 CV (%) 4.7% 5.8% 4.9% 5.5% 5.9% 6.2%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 260 258 290 262 17.34 6.6% Sample 2 1648 1727 1660 1766 1700 48.44 2.8% Sample 3 4379 4465 4565 4726 4533 129.04 2.8% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

23190873

elisa-kits