ELISA/assay

Human KIT / SCFR / CD117 / c Kit ELISA Kit PicoKine®

EK0835

1

Human KIT / SCFR / CD117 / c Kit ELISA Kit PicoKine®

human-kit-scfr-picokine-trade-elisa-kit-ek0835-boster

Picokine ELISA Kits

KIT

369

156 pg/ml - 10,000 pg/ml

Human KIT / SCFR / CD117 / c Kit ELISA Kit PicoKine (96 Tests). Quantitate Human KIT in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Human KIT / SCFR / CD117 / c Kit ELISA Kit PicoKine (96 Tests). Quantitate Human KIT in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

P10721

Expression system for standard: sf21; Immunogen sequence: Q26-T520

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

50 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

ELISA

Human

/antibody/ek0835-2-ELISA-human-kit-scfr-picokine-elisa-kit.jpg

Human KIT/SCFR PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.037 0.195 0.347 0.635 0.871 1.373 1.713 2.092

7/8/14 23:17

3/2/22 2:54

Human KIT / SCFR / CD117 / c Kit ELISA Kit Price PicoKine®

Human KIT/SCFR ELISA Kit, c, KIT ELISA, Bosterbio High Sensitivity

Human KIT / SCFR / CD117 / c Kit ELISA Kit PicoKine (96 Tests). Quantitate Human KIT in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

100

heparin or EDTA

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 313 pg/ml, 156 pg/ml

SCFR (Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

Cancer,Cell Type Markers,Cytokines,Developmental Biology,Germ Cell Markers,Germline Stem Cells,Hematopoietic Progenitors,Immunology,Myeloid,Neuroscience,Neurotransmission,Oncoproteins,Oncoproteins/Suppressors,Organogenesis,Protein Phosphorylation,Receptor Tyrosine Kinases,Receptors / Channels,Reproduction,Signal Transduction,Stem Cells,Surface Molecules,Tags & Cell Markers,Tumor Associated,Tyrosine Kinases

Mast/stem cell growth factor receptor Kit;SCFR;2.7.10.1;Piebald trait protein;PBT;Proto-oncogene c-Kit;Tyrosine-protein kinase Kit;p145 c-kit;v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog;CD117;KIT;SCFR;

Mast/stem cell growth factor receptor Kit

109865 MW

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Mast/stem cell growth factor receptor Kit

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 339 1218 4619 346 1138 4342 Standard deviation 23.39 64.55 314.09 26.98 60.31 329.99 CV (%) 6.9% 5.3% 6.8% 7.8% 5.3% 7.6%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 339 296 321 331 321 16.17 5% Sample 2 1218 1298 1258 1193 1241 39.9 3.2% Sample 3 4619 4300 4189 4585 4423 183.4 4.1% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

19480722

elisa-kits