Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine¬Æ

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine¬Æ

catalog :

EK0831

more info or order :

Boster product webpage

citations: 1

Reference
Saki F, Karamizadeh Z, Honar N, Moravej H, Ashkani Esfahani S, Namvar Shooshtarian M. Association of Plasma Retinol Binding Protein-4 (RBP4) and Sonographic Grading of Fatty Liver in Obese Iranian Children. Hepat Mon. 2012;12:e7103 pubmed publisher

image

image 1 :

Human RBP4 PicoKine ELISA Kit standard curve

product information

sku :

EK0831

status :

name :

Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine¬Æ

url key :

human-rbp4-picokine-trade-elisa-kit-ek0831-boster

product category :

Picokine ELISA Kits

gene name :

RBP4

price :

400

assay range :

312 pg/ml - 20,000 pg/ml

description :

Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine (96 Tests). Quantitate Human RBP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

short description :

Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine (96 Tests). Quantitate Human RBP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P02753

immunogen :

Expression system for standard: NS0; Immunogen sequence: E19-L201

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

20 ng/tube

sample type :

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

application details :

Dilution ratio of 1:20000, concentration in serum is around 28ug/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0831.png

image labels :

Human RBP4 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.029 0.066 0.108 0.215 0.495 1.074 1.866 2.408

created at :

7/8/14 23:17

updated at :

3/2/22 2:54

description before attributes :

Featured Product . Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Human RBP4/Retinol Binding Protein 4 PicoKine Fast ELISA Kit and many others.

meta title :

Human RBP4/Retinol Binding Protein 4 ELISA Kit Price PicoKine¬Æ

meta keyword :

Human RBP4 ELISA Kit, Bosterbio High Sensitivity

meta description :

Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine (96 Tests). Quantitate Human RBP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

rating value :

98.7

anticoagulant :

heparin or EDTA

assay procedure step 1 :

20,000 pg/ml, 10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml

background :

Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It is protein that in humans is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c (Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.

research category :

Atherosclerosis,Cancer,Cardiovascular,Cofactors, Vitamins / Minerals,Diabetes,Diabetes Associated,Heart Disease,Metabolism,Pathways And Processes,Serum Proteins,Signal Transduction,Vitamins / Minerals

synonyms :

Retinol-binding protein 4;Plasma retinol-binding protein;PRBP;RBP;Plasma retinol-binding protein (1-182);Plasma retinol-binding protein (1-181);Plasma retinol-binding protein (1-179);Plasma retinol-binding protein (1-176);RBP4;PRO2222;

gene full name :

Retinol-binding protein 4

molecular weight :

23010 MW

protein function :

Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.

subcellular localization :

Secreted.

protein name :

Retinol-binding protein 4

sequence similarities :

Belongs to the calycin superfamily. Lipocalin family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 621 2874 9419 643 2778 10172 Standard deviation 44.09 227.04 668.74 53.36 247.24 752.72 CV (%) 7.1% 7.9% 7.1% 8.3% 8.9% 7.4%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 621 574 637 603 608 23.39 3.8% Sample 2 2874 2703 2656 2896 2782 104.37 3.7% Sample 3 9419 8739 8492 8429 8769 392.34 4.4% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

23423766

template :

elisa-kits

more info or order :

Boster product webpage