ELISA/assay

Human LOX-1/OLR1 ELISA Kit PicoKine®

EK0824

1

Human LOX-1/OLR1 ELISA Kit PicoKine®

human-lox-1-olr1-picokine-trade-elisa-kit-ek0824-boster

Picokine ELISA Kits

OLR1

369

31.2 pg/ml - 2,000 pg/ml

Human LOX-1/OLR1 ELISA Kit PicoKine (96 Tests). Quantitate Human OLR1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Human LOX-1/OLR1 ELISA Kit PicoKine (96 Tests). Quantitate Human OLR1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

P78380

Expression system for standard: NS0; Immunogen sequence: S61-Q273

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:1, concentration in serum is around 300pg/ml

ELISA

Human

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Human LOX-1/OLR1 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.030 0.162 0.219 0.384 0.694 1.198 1.694 2.032

5/11/14 13:17

3/2/22 2:54

Human LOX-1/OLR1 ELISA Kit Price PicoKine® BosterBio

Human LOX-1/OLR1 ELISA Kit, CLEC8A ELISA Kit, Bosterbio High Sensitivity

Human LOX-1/OLR1 ELISA Kit PicoKine (96 Tests). Quantitate Human OLR1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

98.8

heparin or EDTA

2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml

LOX-1 (lectin-type oxidized LDL receptor 1) also known as OLR1, is a protein that in humans is encoded by the OLR1 gene. LOX-1 is a receptor protein which belongs to the C-type lectin superfamily. The LOX1 gene is mapped to 12p13-p12 by fluorescence in situ hybridization. LOX1 is expressed on the plasma membrane of differentiated macrophages, but not on monocytes. The LOX1 protein acts as a macrophage scavenger receptor. LOX1 expression was detected in all choroidal neovascular membranes, regardless of structure, whereas there was no evidence of LOX1 within the posterior segments of normal eyes. LOX1 plays an active role in the pathogenesis of choroidal neovascularization, especially in ARMD.

Cardiovascular,Cell Biology,Fatty Acids,Lipid Metabolism,Lipids / Lipoproteins,Metabolism,Oxidative Stress,Signal Transduction

Oxidized low-density lipoprotein receptor 1;Ox-LDL receptor 1;C-type lectin domain family 8 member A;Lectin-like oxidized LDL receptor 1;LOX-1;Lectin-like oxLDL receptor 1;hLOX-1;Lectin-type oxidized LDL receptor 1;Oxidized low-density lipoprotein receptor 1, soluble form;OLR1;CLEC8A, LOX1;

Oxidized low-density lipoprotein receptor 1

30959 MW

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria.

Cell membrane; Lipid-anchor. Cell membrane; Single-pass type II membrane protein. Membrane raft. Secreted. A secreted form also exists. Localization to membrane rafts requires palmitoylation.

Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level.

Oxidized low-density lipoprotein receptor 1

Contains 1 C-type lectin domain.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 211 833 90 192 784 Standard deviation 6.86 12.02 64.14 8.46 10.94 69.77 CV (%) 7.8% 5.7% 7.7% 9.4% 5.7% 8.9%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 77 81 83 82 3.96 4.8% Sample 2 211 220 195 196 205 10.5 5.1% Sample 3 833 881 979 973 916 61.9 6.7% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

21364202

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