ELISA/assay

Human KLK8/Kallikrein-8 ELISA Kit PicoKine®

EK0819

1

Human KLK8/Kallikrein-8 ELISA Kit PicoKine®

human-klk8-kallikrein-8-picokine-trade-elisa-kit-ek0819-boster

Picokine ELISA Kits

KLK8

399

156 pg/ml - 10,000 pg/ml

Human KLK8/Kallikrein-8 ELISA Kit PicoKine (96 Tests). Quantitate Human KLK8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Human KLK8/Kallikrein-8 ELISA Kit PicoKine (96 Tests). Quantitate Human KLK8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

O60259

Expression system for standard: NS0; Immunogen sequence: Q29-G260

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:1, concentration in serum is around 600pg/ml

ELISA

Human

/e/k/ek0819_2.png

Human KLK8/Kallikrein-8 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.176 0.268 0.391 0.772 1.300 1.634 2.058

9/17/15 13:05

3/2/22 2:55

Human KLK8/Kallikrein-8 ELISA Kit Price PicoKine® BosterBio

Human KLK8/Kallikrein-8 ELISA Kit, Kallikrein 8 ELISA

Human KLK8/Kallikrein-8 ELISA Kit PicoKine (96 Tests). Quantitate Human KLK8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

100

heparin or EDTA

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

Kallikrein-8 is a protein that in humans is encoded by the KLK8 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in four transcript variants encoding four different isoforms. The isoforms exhibit distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer.

sc 67667 sc 292341 sc 67666 sc 67664 sc 292340

Cancer,Cell Biology,Cytoskeleton / Ecm,Ecm Enzymes,Extracellular Matrix,Invasion/Microenvironment,Proteolysis / Ubiquitin,Proteolytic Enzymes,Signal Transduction

Kallikrein-8;hK8;3.4.21.118;Neuropsin;NP;Ovasin;Serine protease 19;Serine protease TADG-14;Tumor-associated differentially expressed gene 14 protein;KLK8;NRPN, PRSS19, TADG14;UNQ283/PRO322;

Kallikrein-8

28048 MW

Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer- collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury.

Secreted . Cytoplasm . Shows a cytoplasmic distribution in the keratinocytes.

Isoform 1 is predominantly expressed in the pancreas. Isoform 2 is expressed in adult brain and hippocampus. Isoform 1 and isoform 2 are found in fetal brain and placenta. Detected in salivary gland, uterus, thymus, breast, testis and kidney but not in spleen, liver, lung or normal ovarian tissue. Displays an 11.5-fold increase in Alzheimer disease hippocampus compared to controls and is overexpressed in some ovarian carcinomas. Expressed at low levels in normal skin while high levels are found in psoriasis vulgaris, seborrheic keratosis, lichen planus and squamous cell carcinoma skin samples. Expressed in the keratinocytes.

Kallikrein-8

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 217 1843 4442 218 1690 4088 Standard deviation 16.7 108.73 333.15 20.71 118.3 322.95 CV (%) 7.7% 7.5% 7.5% 9.5% 7% 7.9%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 217 203 200 202 205 6.72 3.2% Sample 2 1843 1850 1769 1673 1783 71.38 4% Sample 3 4442 4794 4201 4701 4534 231.75 5.1% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

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