Human MIF ELISA Kit PicoKine¬Æ | Boster EK0813 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human MIF ELISA Kit PicoKine¬Æ

catalog :

EK0813

more info or order :

Boster product webpage

citations: 2

Reference
Sherma N, Borges C, Trenchevska O, Jarvis J, Rehder D, Oran P, et al. Mass Spectrometric Immunoassay for the qualitative and quantitative analysis of the cytokine Macrophage Migration Inhibitory Factor (MIF). Proteome Sci. 2014;12:52 pubmed publisher
Li Y, Zeng Z, Deng S. Study of the relationship between human MIF level, MIF-794CATT5-8 microsatellite polymorphism, and susceptibility of tuberculosis in Southwest China. Braz J Infect Dis. 2012;16:383-6 pubmed publisher

image

image 1 :

Human MIF PicoKine ELISA Kit standard curve

product information

sku :

EK0813

status :

name :

Human MIF ELISA Kit PicoKine¬Æ

url key :

human-mif-picokine-trade-elisa-kit-ek0813-boster

product category :

Picokine ELISA Kits

gene name :

MIF

price :

400

assay range :

156 pg/ml - 10,000 pg/ml

description :

Human MIF ELISA Kit PicoKine (96 Tests). Quantitate Human MIF in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 20pg/ml.

short description :

Human MIF ELISA Kit PicoKine (96 Tests). Quantitate Human MIF in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 20pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

I4AY87

immunogen :

Expression system for standard: E.coli; Immunogen sequence: P2-A115

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<20 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

application details :

Dilution ratio of 1:5, concentration in serum is around 5ng/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0813_1.png

image labels :

Human MIF PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.112 0.198 0.318 0.611 1.100 1.696 2.017

created at :

5/12/14 3:17

updated at :

3/16/22 2:38

meta title :

Human MIF ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human MIF ELISA Kit, GIF/GLIF ELISA, Bosterbio High Sensitivity

meta description :

Human MIF ELISA Kit PicoKine (96 Tests). Quantitate Human MIF in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 20pg/ml.

rating value :

98.7

anticoagulant :

heparin or EDTA

assay procedure step 1 :

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

background :

Macrophage migration inhibitory factor (MIF) is a protein which in humans is encoded by the MIF gene.1, 2 This gene is located to human chromosome 22q11.2.3 It is remarkably small; it has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb.4 This gene encodes a lymphokine that may be involved in cell-mediated immunity, immunoregulation, and inflammation.5 MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein might form a complex in the cytosol near the peripheral plasma membrane, which may possibly indicate a role in integrin signaling pathways. MIF also plays a central role in the toxic response to endotoxemia and possibly septic shock.Macrophage migration inhibitory factor has been reported to interact with COP9 constitutive photomorphogenic homolog subunit 5, CD74, BNIPL, and CXCR4.

competitor equivalent skus :

sc 16966 sc 16965 sc 48241 sc 20121 sc 271631 sc 47159 sc 53915 sc 130329

research category :

Macrophage / Inflamm.

synonyms :

Macrophage migration inhibitory factor ;MIF ;

gene full name :

macrophage migration inhibitory factor (glycosylation-inhibiting factor)

molecular weight :

12476 MW

protein name :

Macrophage migration inhibitory factor

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 280 1655 4559 305 1694 4885 Standard deviation 12.04 66.2 206.35 16.47 77.92 307.75 CV (%) 4.3% 4% 4.5% 5.4% 4.6% 6.3%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 280 266 256 273 268 8.87 3.3% Sample 2 1655 1562 1783 1751 1687 86.54 5.1% Sample 3 4559 4531 3899 4612 4400 290.85 6.6% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

22846129, 25328446

template :

elisa-kits

more info or order :

Boster product webpage