Human MICA ELISA Kit PicoKine¬Æ | Boster EK0812 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human MICA ELISA Kit PicoKine¬Æ

catalog :

EK0812

more info or order :

Boster product webpage

citations: 1

Reference
Li Y, Xia B, Lu M, Ge L, Zhang X. MICB0106 gene polymorphism is associated with ulcerative colitis in central China. Int J Colorectal Dis. 2010;25:153-9 pubmed publisher

image

image 1 :

Human MICA PicoKine ELISA Kit standard curve

product information

sku :

EK0812

status :

name :

Human MICA ELISA Kit PicoKine¬Æ

url key :

human-mica-picokine-trade-elisa-kit-ek0812-boster

product category :

Picokine ELISA Kits

gene name :

MICA

price :

400

assay range :

62.5 pg/ml - 4,000 pg/ml

description :

Human MICA ELISA Kit PicoKine (96 Tests). Quantitate Human MICA in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml.

short description :

Human MICA ELISA Kit PicoKine (96 Tests). Quantitate Human MICA in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

Q29983

immunogen :

Expression system for standard: NS0; Immunogen sequence: E24-W307

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates and serum.

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0812.png

image labels :

Human MICA PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.114 0.165 0.287 0.470 0.792 1.228 1.945

created at :

5/12/14 3:17

updated at :

3/2/22 2:54

meta title :

Human MICA ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human MICA ELISA Kit, FLJ60820 ELISA Kit, Bosterbio High Sensitivity

meta description :

Human MICA ELISA Kit PicoKine (96 Tests). Quantitate Human MICA in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml.

rating value :

100

assay procedure step 1 :

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

background :

MHC class I polypeptide-related sequence A is a protein that in humans is encoded by the MICA gene.1 The MICA gene encodes a 383-amino acid polypeptide with a predicted mass of 43 kD.The MICA and MICB genes occur in a 200-kb region spanning the TNFA and TNFB cluster at 6p21.3.2 MICA and the closely related MICB were recognized by intestinal epithelial T cells expressing diverse V-delta-1 gamma/delta TCRs.3 The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is further distinguished by its unusual exon-intron organization and preferential expression in fibroblasts and epithelial cells. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. MICA and other members of this family may have been selected for specialized functions that are either ancient or derived from those of typical MHC class I genes, in analogy to some of the nonclassic mouse H-2 genes.

competitor equivalent skus :

sc 23870

research category :

Adaptive Immunity,Class I,Immunology,Innate Immunity,Nk Cells

synonyms :

MHC class I polypeptide-related sequence A;MIC-A;MICA ;PERB11.1 ;

gene full name :

MHC class I polypeptide-related sequence A

molecular weight :

42915 MW

protein function :

Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T- cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.

subcellular localization :

Cell membrane ; Single- pass type I membrane protein . Cytoplasm . Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum.

tissue specificity :

Widely expressed with the exception of the central nervous system where it is absent. Expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within the medulla of normal thymus. In skin, expressed mainly in the keratin layers, basal cells, ducts and follicles. Also expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In thyomas, overexpressed in cortical and medullar epithelial cells. Tumors expressing MICA display increased levels of gamma delta T-cells.

protein name :

MHC class I polypeptide-related sequence A

sequence similarities :

Belongs to the MHC class I family. MIC subfamily.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 151 574 1670 165 570 1549 Standard deviation 11.17 41.32 120.24 14.02 46.74 119.27 CV (%) 7.4% 7.2% 7.2% 8.5% 8.2% 7.7%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 151 138 157 144 147 7.15 4.8% Sample 2 574 643 534 540 572 43.33 7.5% Sample 3 1670 1895 1663 1861 1772 106.45 6% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

19662431

template :

elisa-kits

more info or order :

Boster product webpage