Human Mer ELISA Kit PicoKine¬Æ | Boster EK0810 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Mer ELISA Kit PicoKine¬Æ

catalog :

EK0810

more info or order :

Boster product webpage

citations: 1

Reference
Zhu H, Sun X, Zhu L, Hu F, Shi L, Li Z, et al. The expression and clinical significance of different forms of Mer receptor tyrosine kinase in systemic lupus erythematosus. J Immunol Res. 2014;2014:431896 pubmed publisher

image

image 1 :

Human Mer PicoKine ELISA Kit standard curve

product information

sku :

EK0810

status :

name :

Human Mer ELISA Kit PicoKine¬Æ

url key :

human-mer-picokine-trade-elisa-kit-ek0810-boster

product category :

Picokine ELISA Kits

gene name :

MERTK

price :

430

assay range :

156 pg/ml - 10,000 pg/ml

description :

Human Mer ELISA Kit PicoKine (96 Tests). Quantitate Human MERTK in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml.

short description :

Human Mer ELISA Kit PicoKine (96 Tests). Quantitate Human MERTK in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

Q12866

immunogen :

Expression system for standard: NS0; Immunogen sequence: R26-A499

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates and serum.

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0810.png

image labels :

Human Mer PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.065 0.143 0.201 0.297 0.595 1.082 1.584 2.067

created at :

5/12/14 3:17

updated at :

3/2/22 2:54

meta title :

Human Mer ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human Mer ELISA Kit, MERTK/cMER ELISA, Bosterbio High Sensitivity

meta description :

Human Mer ELISA Kit PicoKine (96 Tests). Quantitate Human MERTK in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml.

rating value :

100

assay procedure step 1 :

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

background :

Proto-oncogene tyrosine-protein kinase MER is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Its gene is mapped to chromosome 2q14.1. Mer encodes a 984-amino acid protein with a calculated molecular mass of 109 kD. It is expressed in numerous neoplastic B- and T-cell lines. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP)

competitor equivalent skus :

sc 66399 sc 66398 sc 53985 sc 53984 sc 66396

research category :

Intracellular Signaling,Kinases,Neuroscience,Neurotransmission,Protein Phosphorylation,Receptor Tyrosine Kinases,Sensory System,Signal Transduction,Tyrosine Kinases,Visual System

synonyms :

Tyrosine-protein kinase Mer;2.7.10.1;Proto-oncogene c-Mer;Receptor tyrosine kinase MerTK;MERTK;MER;

gene full name :

Tyrosine-protein kinase Mer

molecular weight :

110249 MW

protein function :

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll- like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

subcellular localization :

Membrane ; Single-pass type I membrane protein .

tissue specificity :

Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. Highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver.

protein name :

Tyrosine-protein kinase Mer

sequence similarities :

Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 236 1665 4937 217 1701 4920 Standard deviation 14.63 79.92 197.48 13.67 105.46 221.4 CV (%) 6.2% 4.8% 4% 6.2%% 6.2% 4.5%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 236 266 272 241 253 15.49 6.1% Sample 2 1665 1842 1731 1814 1763 69.76 3.9% Sample 3 4937 4427 4544 5104 4753 277.04 5.8% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

polyclonal antibody from goat polyclonal antibody from goat

normal range citations :

24741600

template :

elisa-kits

more info or order :

Boster product webpage